Qiao Yi-Jun, Zhang Jia-Jiao, Shang Jia-Huan, Zhu Hong-Tao, Wang Dong, Yang Chong-Ren, Zhang Ying-Jun
State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming Institute of Botany, Chinese Academy of Sciences Kunming 650201 People's Republic of China
University of Chinese Academy of Sciences Beijing 100049 People's Republic of China.
RSC Adv. 2019 Jul 2;9(36):20557-20564. doi: 10.1039/c9ra02110h. eCollection 2019 Jul 1.
Continuous cropping obstacle, mainly caused by microorganisms and organic components in soil, has become a serious problem for the plantation of (Araliaceae) due to the rapidly increased demands of this famous herbal medicine in recent decades. The rhizosphere soils cultivated with 3-year-old healthy and ill notoginseng were chemically investigated by gas chromatography-mass spectrometry (GC-MS) and compared with the corresponding soils without the plantation of notoginseng. Totally 47 liposoluble components were identified. Furthermore, the multiple statistical analysis showed that these constituents were qualitatively and quantitatively associated with the differences between the cultivated soil with and the uncultivated soil. Among them, neophytadiene (4), d-α-tocopherol (38), (3β,22,24)-ergosta-5,22-dien-3-ol (39), (3β,24)-ergost-5-en-3-ol (40), stigmasta-5,22-dien-3-ol (41), stigmast-4-en-3-one (44) and (5α)-stigmastane-3,6-dione (47) contributed most to the significant differences between the cultivated and uncultivated soils, whereas cyclopentadecane (3), octadecanoic acid methyl ester (16), docosanoic acid ethyl ester (31), nonacosane (34), 38 and 39 were found in much higher amount in the soils with ill as compared to the case of those with the healthy . On the other hand, liposoluble components in different cultivation areas were of great diversity; however, they were able to remain relatively consistent across the overall trend of differential substances.
连作障碍主要由土壤中的微生物和有机成分引起,近几十年来,由于对这种名贵中药材的需求迅速增加,连作障碍已成为三七种植的一个严重问题。采用气相色谱-质谱联用仪(GC-MS)对种植3年的健康三七和患病三七的根际土壤进行了化学分析,并与未种植三七的相应土壤进行了比较。共鉴定出47种脂溶性成分。此外,多元统计分析表明,这些成分在定性和定量上与种植三七的土壤和未种植三七的土壤之间的差异有关。其中,新植二烯(4)、d-α-生育酚(38)、(3β,22,24)-麦角甾-5,22-二烯-3-醇(39)、(3β,24)-麦角甾-5-烯-3-醇(40)、豆甾-5,22-二烯-3-醇(41)、豆甾-4-烯-3-酮(44)和(5α)-豆甾烷-3,6-二酮(47)对种植土壤和未种植土壤之间的显著差异贡献最大,而与健康三七相比,患病三七土壤中检测到的环十五烷(3)、十八烷酸甲酯(16)、二十二烷酸乙酯(31)、二十九烷(34)、38和39的含量要高得多。另一方面,不同种植区的脂溶性成分具有很大的多样性;然而,它们在差异物质的总体趋势上能够保持相对一致。