Huang Wei, Hou Xingyu, Tong Yukui, Tian Miaomiao
Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, College of Chemistry and Chemical Engineering, Harbin Normal University Harbin 150025 China
RSC Adv. 2019 Feb 12;9(10):5394-5401. doi: 10.1039/c9ra00511k. eCollection 2019 Feb 11.
Boronate-affinity magnetic hollow molecularly imprinted polymers (B-MhMIPs) were prepared with sialic acid (SA) as the template, 3-aminophenylboronic acid (APBA) as the functional monomer and glycidilmethacrylate (GMA) as the co-monomer to chemisorb FeO nanoparticles. Furthermore, the hollow structure made the nanoparticles have more binding sites at both internal and external surfaces, which can facilitate the removal of template molecules from polymers and enhance the adsorption abilities towards SA. After optimizing, the adsorption pH was controlled at 4.0, and this was different from most -diol-containing compounds. Under the optimal conditions, the limit of detection for SA was 0.025 μg mL ( = 3). This method was applied to analyze serum samples with different spiked levels, and the recoveries of the SA were in the range of 70.9-106.2%. These results confirmed the superiority of the B-MhMIPs for selective and efficient enrichment of trace SA from complex matrices.
以唾液酸（SA）为模板、3-氨基苯硼酸（APBA）为功能单体、甲基丙烯酸缩水甘油酯（GMA）为共聚单体，制备了用于化学吸附FeO纳米颗粒的硼酸亲和磁性中空分子印迹聚合物（B-MhMIPs）。此外，中空结构使纳米颗粒在内表面和外表面都有更多的结合位点，这有助于从聚合物中去除模板分子，并增强对SA的吸附能力。优化后，吸附pH控制在4.0，这与大多数含二醇化合物不同。在最佳条件下，SA的检测限为0.025 μg mL（n = 3）。该方法用于分析不同加标水平的血清样品，SA的回收率在70.9%至106.2%之间。这些结果证实了B-MhMIPs在从复杂基质中选择性高效富集痕量SA方面的优越性。
