Feng Xiaotong, Ashley Jon, Zhou Tongchang, Halder Arnab, Sun Yi
Department of Micro- and Nanotechnology, Technical University of Denmark Ørsteds Plads, DK-2800 Kgs, Lyngby Denmark
RSC Adv. 2018 Jan 9;8(5):2365-2372. doi: 10.1039/c7ra11507e.
Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, -phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 μM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.
组胺是一种天然存在于许多人体细胞中的生物胺。它也是一种污染物，主要存在于变质食品中。食用含有高浓度组胺的食物可能会导致类似过敏的食物中毒。因此，迫切需要能够常规筛查组胺的分析方法。在本文中，我们开发了一种简便且经济高效的基于分子印迹聚合物（MIP）的荧光测定法来直接定量组胺。使用改良的固相合成方法合成了组胺特异性MIP纳米颗粒（nanoMIPs）。然后将它们固定在微孔板的孔中，以结合水性样品中的组胺。结合后，使用邻苯二甲醛（OPA）标记结合的组胺，将结合事件转化为荧光信号。获得的组胺校准曲线在1.80至44.98 μM范围内呈线性相关，检测限为1.80 μM。该方法成功用于检测强化奶粉中的组胺，回收率超过85％。
