Khan M Z H, Ahommed M S, Daizy M
Dept. of Chemical Engineering, Jashore University of Science and Technology Jashore 7408 Bangladesh
Department of Chemistry, Graduate School of Science, Tohoku University Aoba-ku Sendai 980-8578 Japan.
RSC Adv. 2020 Sep 30;10(59):36147-36154. doi: 10.1039/d0ra06806c. eCollection 2020 Sep 28.
An innovative biosensor assembly relying on glassy carbon electrodes modified with nanocomposites consisting of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) as a host matrix with functionalized gold nanoparticles (GCE/PEDOT:PSS-AuNPs) is presented for the selective and sensitive detection of xanthine (XA). The developed sensor was successfully applied for the quantification of XA in the presence of significant interferents like hypoxanthine (HXA) and uric acid (UA). Different spectroscopy and electron microscopy analyses were done to characterize the as-prepared nanocomposite. Calibration responses for the quantification of XA was linear from 5.0 × 10 to 1.0 × 10 M ( = 0.994), with a detection limit as low as 3.0 × 10 (S/N = 3). Finally, the proposed sensor was applied for the analyses of XA content in commercial fish and meat samples and satisfactory recovery percentage was obtained.
本文提出了一种创新的生物传感器组件，该组件基于玻碳电极，电极用由聚（3,4 - 乙撑二氧噻吩）聚苯乙烯磺酸盐（PEDOT:PSS）作为主体基质与功能化金纳米颗粒组成的纳米复合材料进行修饰（GCE/PEDOT:PSS - AuNPs），用于选择性和灵敏地检测黄嘌呤（XA）。所开发的传感器成功应用于在存在次黄嘌呤（HXA）和尿酸（UA）等显著干扰物的情况下对XA进行定量。进行了不同的光谱和电子显微镜分析以表征所制备的纳米复合材料。XA定量的校准响应在5.0×10至1.0×10 M范围内呈线性（ = 0.994），检测限低至3.0×10（S/N = 3）。最后，将所提出的传感器应用于商业鱼类和肉类样品中XA含量的分析，并获得了令人满意的回收率。
