Li Li, Gu Ning, Dong Huijuan, Li Bingsheng, T V G Kenneth
School of Life Sciences and Technology, Harbin Institute of Technology Harbin 150080 China.
State Key Laboratory of Robotics and Systems, Harbin Institute of Technology Harbin 150080 China
RSC Adv. 2020 Dec 17;10(72):44593-44600. doi: 10.1039/d0ra07344j. eCollection 2020 Dec 9.
In this work, an acoustic standing wave field (ASWF) is used to simulate the space environment, which shows characteristics such as microgravity and the absence of containment and contact. Zebrafish embryos, used as the species under study in this work, were raised within the acoustic field by the authors, allowing the biological effects on such early zebrafish embryos, at each developmental stage and within the ASWF creating the acoustic levitation (AL) technology used, to be studied. In this way, the biological safety of thee specimens, simulating the space environment, could be carefully evaluated. Some important indexes of the process of zebrafish development, such as mortality, malformation rate, hatching rate, voluntary movement and heart rate were detected and analyzed. It has been found that the ASWF exerted considerable influence on the zebrafish embryos at the early development stage, influencing features such as the cleavage, blastula and gastrul stage, over the period 0-8 hour post fertilization (hpf). The zebrafish appear to show some features of teratogenesis, as well as lethal effects and a significant decrease of the hatching rate, after being treated by using the AL that was applied. Furthermore, it was observed that voluntary movements and the embryo heart rates apparently increased under these conditions. However, as the development of the embryo progressed into the bursa pharyngea stage (at 24-32 hpf), the influence of the ASWF creating the AL on zebrafish seemed almost to be insignificant, as there was no obvious difference between the characteristics of the experimental group and the control group. The experiment carried out has provided a scientific reference for the application of AL in this field, allowing the biological safety aspects of such zebrafish embryo development within a space environment to be evaluated.
在这项工作中，使用声学驻波场（ASWF）来模拟空间环境，该环境呈现出微重力以及无容器和无接触等特征。本研究中作为研究对象的斑马鱼胚胎，由作者在声场中进行培育，从而能够研究在每个发育阶段以及使用声学悬浮（AL）技术所产生的ASWF环境下，对早期斑马鱼胚胎的生物学效应。通过这种方式，可以仔细评估模拟空间环境的这些样本的生物安全性。检测并分析了斑马鱼发育过程中的一些重要指标，如死亡率、畸形率、孵化率、自主运动和心率。研究发现，在受精后0至8小时（hpf）期间，ASWF对早期发育阶段的斑马鱼胚胎产生了相当大的影响，影响了诸如卵裂、囊胚和原肠胚阶段等特征。使用所应用的AL处理后，斑马鱼似乎表现出一些致畸特征、致死效应以及孵化率的显著下降。此外，观察到在这些条件下自主运动和胚胎心率明显增加。然而，随着胚胎发育进入咽囊阶段（24至32 hpf），产生AL的ASWF对斑马鱼的影响似乎几乎微不足道，因为实验组和对照组的特征之间没有明显差异。所进行的实验为AL在该领域的应用提供了科学参考，使得能够评估在空间环境中此类斑马鱼胚胎发育的生物安全性方面。
