Sundvik Maria, Nieminen Heikki J, Salmi Ari, Panula Pertti, Hæggström Edward
Neuroscience Center and Department of Anatomy, POB 63, 00014 University of Helsinki, Finland.
Electronics Research Laboratory and Department of Physics, POB 64, 00014 University of Helsinki, Finland.
Sci Rep. 2015 Sep 4;5:13596. doi: 10.1038/srep13596.
Acoustic levitation provides potential to characterize and manipulate material such as solid particles and fluid in a wall-less environment. While attempts to levitate small animals have been made, the biological effects of such levitation have been scarcely documented. Here, our goal was to explore if zebrafish embryos can be levitated (peak pressures at the pressure node and anti-node: 135 dB and 144 dB, respectively) with no effects on early development. We levitated the embryos (n = 94) at 2-14 hours post fertilization (hpf) for 1000 (n = 47) or 2000 seconds (n = 47). We compared the size and number of trunk neuromasts and otoliths in sonicated samples to controls (n = 94), and found no statistically significant differences (p > 0.05). While mortality rate was lower in the control group (22.3%) compared to that in the 1000 s (34.0%) and 2000 s (42.6%) levitation groups, the differences were statistically insignificant (p > 0.05). The results suggest that acoustic levitation for less than 2000 sec does not interfere with the development of zebrafish embryos, but may affect mortality rate. Acoustic levitation could potentially be used as a non-contacting wall-less platform for characterizing and manipulating vertebrae embryos without causing major adverse effects to their development.
声悬浮技术为在无壁环境中表征和操控诸如固体颗粒及流体等物质提供了可能。尽管已有尝试对小动物进行悬浮，但此类悬浮的生物学效应鲜有文献记载。在此，我们的目标是探究斑马鱼胚胎能否在不影响早期发育的情况下实现悬浮（压力节点和反节点处的峰值压力分别为135分贝和144分贝）。我们在受精后2至14小时（hpf）对胚胎（n = 94）进行了1000秒（n = 47）或2000秒（n = 47）的悬浮处理。我们将超声处理样本中的躯干神经丘和耳石的大小及数量与对照组（n = 94）进行比较，未发现统计学上的显著差异（p > 0.05）。虽然对照组的死亡率（22.3%）低于1000秒（34.0%）和2000秒（42.6%）悬浮组，但差异无统计学意义（p > 0.05）。结果表明，2000秒以内的声悬浮不会干扰斑马鱼胚胎的发育，但可能影响死亡率。声悬浮技术有可能用作一种非接触式无壁平台，用于表征和操控脊椎动物胚胎，而不会对其发育造成重大不利影响。
