Zhang Yi, Gao Zhiyong, Yang Xue, Yang Genqing, Chang Jiuli, Jiang Kai
School of Chemistry and Chemical Engineering, Henan Normal University Henan Xinxiang 453007 P. R. China
School of Laboratory Medicine, Xinxiang Medical University Henan Xinxiang 453003 P. R. China.
RSC Adv. 2019 Feb 19;9(11):6084-6093. doi: 10.1039/c9ra00360f. eCollection 2019 Feb 18.
Highly fluorescent carbon dots (CDs) were synthesized through facile hydrothermal carbonization and ethylenediamine passivation of an easily available prawn shell precursor. The as-prepared CDs exhibit high water solubility, wavelength-tunable fluorescence with quantum yield up to 68.9%, high photostability and resistance against biomolecules, thus enabling the application as viable fluorescent nanoprobes for detection of guest quenchers. The fluorescence of the CDs can be effectively quenched by clomifene citrate (CC, a common drug for infertility) through static quenching, and therefore can serve as a simple and efficient fluorescent nanoprobe for determination of CC with wide linear range (0.25-10 μg mL) and low detection limit (0.2 μg mL). The CDs also showed low cytotoxicity, which enables the safe and accurate fluorescent detection of spiked CC in human serum, demonstrating their potential as a credible fluorescent CC nanoprobe in clinical examination.
通过对易于获得的虾壳前驱体进行简便的水热碳化和乙二胺钝化，合成了高荧光碳点（CDs）。所制备的CDs具有高水溶性、量子产率高达68.9%的波长可调荧光、高光稳定性和对生物分子的抗性，从而使其能够作为可行的荧光纳米探针用于检测客体猝灭剂。CDs的荧光可通过柠檬酸氯米芬（CC，一种常用的不孕症药物）通过静态猝灭有效地猝灭，因此可作为一种简单有效的荧光纳米探针用于测定CC，具有宽线性范围（0.25 - 10 μg mL）和低检测限（0.2 μg mL）。CDs还表现出低细胞毒性，这使得能够对人血清中加标的CC进行安全准确的荧光检测，证明了它们作为临床检查中可靠的荧光CC纳米探针的潜力。
