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通过整合辅酶A连接酶和来自……的III型聚酮合酶酶促合成2-羟基-4-喹啉嗪-4-酮支架

Enzymatic synthesis of 2-hydroxy-4-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from .

作者信息

Wang Juan, Ding Ning, Wu Yun, Shi Xiaoping, Qi Bowen, Liu Xiao, Wang Xiaohui, Li Jun, Tu Pengfei, Shi Shepo

机构信息

Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029 China

出版信息

RSC Adv. 2020 Jun 22;10(40):23566-23572. doi: 10.1039/d0ra04133e. eCollection 2020 Jun 19.

DOI:10.1039/d0ra04133e
PMID:35517366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9054772/
Abstract

2-Hydroxy-4-quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus MT-12, malonyl-CoA synthase (AtMatB) from , and a type III polyketide synthase (HsPKS3) from Chinese club moss . The findings paved the way to produce these kinds of structurally interesting alkaloids by engineered microorganisms.

摘要

通过整合三种酶，即来自内生真菌MT-12的苯乙酸辅酶A连接酶（PcPCL）、来自拟南芥的丙二酰辅酶A合酶（AtMatB）和来自中华卷柏的III型聚酮合酶（HsPKS3），酶促合成了2-羟基-4-喹唑啉-4-酮支架。这些发现为通过工程微生物生产这类结构有趣的生物碱铺平了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/80f28da21ff3/d0ra04133e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/4c1ead2cd182/d0ra04133e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/e7256d94acba/d0ra04133e-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/4d63f5599a4a/d0ra04133e-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/80f28da21ff3/d0ra04133e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/4c1ead2cd182/d0ra04133e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/e7256d94acba/d0ra04133e-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/4d63f5599a4a/d0ra04133e-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f63b/9054772/80f28da21ff3/d0ra04133e-f2.jpg

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