Haynes Anna Z, Levine Mindy
University of Rhode Island, Department of Chemistry 140 Flagg Road Kingston RI 02881 USA.
Ariel University, Department of Chemical Sciences 65 Ramat HaGolan Street Ariel Israel
RSC Adv. 2020 Jul 1;10(42):25108-25115. doi: 10.1039/d0ra03485a. eCollection 2020 Jun 29.
Reported herein is the detection of anabolic steroids through the use of cyclodextrin-promoted interactions between the analyte of interest and a high quantum yield fluorophore, which lead to measurable, analyte-specific changes in the fluorophore emission signal. By using a variety of β-cyclodextrin derivatives (unmodified β-cyclodextrin, methyl-β-cyclodextrin, and 2-hydroxypropyl-β-cyclodextrin) in combination with high quantum yield fluorophore rhodamine 6G, we detected five anabolic steroid analytes with 100% differentiation between structurally similar analytes and micromolar level limits of detection. Overall, these results show significant potential in the development of practical, fluorescence-based steroid detection devices.
本文报道了通过利用环糊精促进目标分析物与高量子产率荧光团之间的相互作用来检测合成代谢类固醇，这种相互作用会导致荧光团发射信号产生可测量的、分析物特异性变化。通过将多种β-环糊精衍生物（未修饰的β-环糊精、甲基-β-环糊精和2-羟丙基-β-环糊精）与高量子产率荧光团罗丹明6G结合使用，我们检测到了五种合成代谢类固醇分析物，在结构相似的分析物之间实现了100%的区分，检测限达到微摩尔水平。总体而言，这些结果显示出在开发实用的基于荧光的类固醇检测设备方面具有巨大潜力。
