Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini street, 11562 Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini street, 11562 Cairo, Egypt; Chemistry Department, School of Pharmacy and Pharmaceutical Industries, Badr University in Cairo, Badr City, 11829 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117237. doi: 10.1016/j.saa.2019.117237. Epub 2019 Jun 3.
Inclusion complexes of β-cyclodextrin with Estradiol valerate (EST) or Norethisterone acetate (NOR) have been utilized for synchronous fluorescence spectrofluorimetry. β-cyclodextrin improves fluorescence intensity as well as water solubility of the studied drugs. Samples in aqueous medium adjusted with ammonia were used in synchronous fluorescence to resolve the overlapped emission spectra. The effects of β-cyclodextrin concentration and Δ λ have been optimized for sensitive and selective analysis of EST and NOR binary mixture. Synchronous fluorescence intensity of the two drugs is measured at Δ λ of 70 nm. EST and NOR can be simultaneously determined at 230 nm and 270 nm, respectively. Calibration curves were linear over the ranges of 0.5-6.0 μg mL and 0.2-2.0 μg mL for EST and NOR, respectively. Official guidelines were followed to estimate the validation parameters of the proposed method. The detection limits were 0.08 μg mL for EST and 0.007 μg mL for NOR. The proposed method was successfully used for the analysis of EST and NOR in their commercial preparations and the obtained results revealed statistical agreement with those obtained by application of the reported method for both drugs. The proposed method is compared favorably to previously published method in terms of simplicity and hazardous solvent consumption.
β-环糊精与戊酸雌二醇（EST）或醋酸炔诺酮（NOR）的包合物已被用于同步荧光分光光度法。β-环糊精提高了研究药物的荧光强度和水溶性。在氨调节的水相介质中使用样品进行同步荧光，以解析重叠的发射光谱。优化了β-环糊精浓度和Δ λ，以实现对 EST 和 NOR 二元混合物的灵敏和选择性分析。两种药物的同步荧光强度在Δ λ为 70nm 时进行测量。EST 和 NOR 可以分别在 230nm 和 270nm 处同时测定。对于 EST 和 NOR，校准曲线在 0.5-6.0μgmL 和 0.2-2.0μgmL 的范围内呈线性。遵循官方指南来估计所提出方法的验证参数。EST 的检测限为 0.08μgmL，NOR 的检测限为 0.007μgmL。该方法成功地用于其商业制剂中 EST 和 NOR 的分析，并且所得结果与应用两种药物的报道方法获得的结果具有统计学一致性。与之前发表的方法相比，该方法在简单性和有害溶剂消耗方面具有优势。
