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结合胆固醇的方式不止一种：通过核糖体展示筛选出的产气荚膜梭菌溶素O膜结合结构域的非典型变体

More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display.

作者信息

Šakanović Aleksandra, Kranjc Nace, Omersa Neža, Podobnik Marjetka, Anderluh Gregor

机构信息

Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry Ljubljana Slovenia

Biosciences Doctoral Program, Biotechnical Faculty, University of Ljubljana Ljubljana Slovenia.

出版信息

RSC Adv. 2020 Oct 21;10(63):38678-38682. doi: 10.1039/d0ra06976k. eCollection 2020 Oct 15.

DOI:10.1039/d0ra06976k
PMID:35517550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9057304/
Abstract

Herein, we report a high-throughput approach for the selection of peripheral protein domains that bind specifically to cholesterol in lipid membranes. We discovered variants of perfringolysin O, with non-conserved amino acid substitutions at regions crucial for cholesterol recognition, demonstrating an unprecedented amino acid sequence variability with binding ability for cholesterol. The developed approach provides an effective platform for a comprehensive study of protein lipid interactions.

摘要

在此，我们报告了一种高通量方法，用于筛选在脂质膜中与胆固醇特异性结合的外周蛋白结构域。我们发现了产气荚膜梭菌溶血素O的变体，其在对胆固醇识别至关重要的区域存在非保守氨基酸取代，这表明其氨基酸序列变异性前所未有的同时仍具有胆固醇结合能力。所开发的方法为全面研究蛋白质-脂质相互作用提供了一个有效的平台。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/8a8cb8109709/d0ra06976k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/f0e71e749b3a/d0ra06976k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/e0ddb41a6093/d0ra06976k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/a1768781d37d/d0ra06976k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/8a8cb8109709/d0ra06976k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/f0e71e749b3a/d0ra06976k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/e0ddb41a6093/d0ra06976k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/a1768781d37d/d0ra06976k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/681c/9057304/8a8cb8109709/d0ra06976k-f3.jpg

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More than one way to bind to cholesterol: atypical variants of membrane-binding domain of perfringolysin O selected by ribosome display.结合胆固醇的方式不止一种：通过核糖体展示筛选出的产气荚膜梭菌溶素O膜结合结构域的非典型变体
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本文引用的文献

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J Am Chem Soc. 2020 Apr 15;142(15):6891-6895. doi: 10.1021/jacs.0c01313. Epub 2020 Mar 31.
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Design of Protein Logic Gate System Operating on Lipid Membranes.基于脂膜的蛋白质逻辑门系统设计。
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Synthetic Protein Scaffolding at Biological Membranes.生物膜上的合成蛋白支架。
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Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action.氯胺酮通过星形胶质细胞特异性重塑质膜胆固醇组成来表明其抗抑郁作用的新机制。
Sci Rep. 2019 Jul 29;9(1):10957. doi: 10.1038/s41598-019-47459-z.
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Emerging Diversity in Lipid-Protein Interactions.脂质-蛋白质相互作用的新多样性。
Chem Rev. 2019 May 8;119(9):5775-5848. doi: 10.1021/acs.chemrev.8b00451. Epub 2019 Feb 13.
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Combination of ribosome display and next generation sequencing as a powerful method for identification of affibody binders against β-lactamase CTX-M15.核糖体展示和下一代测序的结合作为一种强大的方法，用于鉴定针对β-内酰胺酶 CTX-M15 的亲和体结合物。
N Biotechnol. 2019 May 25;50:60-69. doi: 10.1016/j.nbt.2019.01.004. Epub 2019 Jan 8.
7
Listeriolysin O Binding Affects Cholesterol and Phospholipid Acyl Chain Dynamics in Fluid Cholesterol-Rich Bilayers.李斯特菌溶血素 O 结合对富含胆固醇流体双层中胆固醇和磷脂酰基链动力学的影响。
Chemistry. 2018 Sep 20;24(53):14220-14225. doi: 10.1002/chem.201802575. Epub 2018 Aug 28.
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F NMR studies provide insights into lipid membrane interactions of listeriolysin O, a pore forming toxin from Listeria monocytogenes.F NMR 研究为李斯特菌溶血素 O（一种来自李斯特菌的孔形成毒素）与脂质膜相互作用提供了深入了解。
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Mechanistic Insights into the Cholesterol-dependent Binding of Perfringolysin O-based Probes and Cell Membranes.关于产气荚膜梭菌毒素 O 基于探针与细胞膜胆固醇依赖性结合的机制研究。
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