Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Trends Biotechnol. 2020 Apr;38(4):432-446. doi: 10.1016/j.tibtech.2019.10.009. Epub 2019 Nov 9.
Protein scaffolding is a natural phenomenon whereby proteins colocalize into macromolecular complexes via specific protein-protein interactions. In the case of metabolic enzymes, protein scaffolding drives metabolic flux through specific pathways by colocalizing enzyme active sites. Synthetic protein scaffolding is increasingly used as a mechanism to improve product specificity and yields in metabolic engineering projects. To date, synthetic scaffolding has focused primarily on soluble enzyme systems, but many metabolic pathways for high-value secondary metabolites depend on membrane-bound enzymes. The compositional diversity of biological membranes and general challenges associated with modifying membrane proteins complicate scaffolding with membrane-requiring enzymes. Several recent studies have introduced new approaches to protein scaffolding at membrane surfaces, with notable success in improving product yields from specific metabolic pathways.
蛋白质支架是一种自然现象，其中蛋白质通过特定的蛋白质-蛋白质相互作用而聚集到大分子复合物中。在代谢酶的情况下，蛋白质支架通过将酶活性位点聚集在一起，驱动特定途径的代谢通量。合成蛋白质支架越来越多地被用作提高代谢工程项目中产物特异性和产量的机制。迄今为止，合成支架主要集中在可溶性酶系统上，但许多高价值次生代谢物的代谢途径依赖于膜结合酶。生物膜的组成多样性以及与修饰膜蛋白相关的一般挑战使得需要膜的酶的支架复杂化。最近的几项研究引入了在膜表面进行蛋白质支架的新方法，在提高特定代谢途径的产物产量方面取得了显著成功。
