Xie Yang-Guo, Yan Rong, Zhong Xianglong, Piao Huang, Muhammad Ishaq, Ke Xisong, Yan Shikai, Guo Yuanqiang, Jin Hui-Zi, Zhang Wei-Dong
Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University Shanghai 200240 China
Institute of Interdisciplinary Sciences, Shanghai University of Tradictional Chinese Medicine Shanghai 201203 China.
RSC Adv. 2019 Mar 21;9(16):9235-9242. doi: 10.1039/c9ra00347a. eCollection 2019 Mar 15.
Six rare guaiane-type sesquiterpene dimers xylopins A-F, having three different connecting modes through two direct C-C bonds, were isolated from the roots of . Their absolute configurations were established by NOESY analysis, Cu Kα X-ray crystallography, and experimental and calculated electronic circular dichroism spectra. Flow cytometry demonstrated the fact that compound 6 arrested the cell cycle at G2 phase and concentration-dependently induced apoptosis of DU145 cells. Furthermore, the EPT2-TGC cell model, zebrafish study and western blot analysis illustrated compound 6 could induce apoptosis by efficiently inhibiting the Wnt/β-catenin signaling pathway decreasing the expression of β-catenin.
从……的根部分离出六种罕见的愈创木烷型倍半萜二聚体木脂素A-F，它们通过两个直接的碳-碳键具有三种不同的连接方式。通过NOESY分析、Cu Kα X射线晶体学以及实验和计算的电子圆二色光谱确定了它们的绝对构型。流式细胞术证明化合物6使细胞周期停滞在G2期，并浓度依赖性地诱导DU145细胞凋亡。此外，EPT2-TGC细胞模型、斑马鱼研究和蛋白质印迹分析表明化合物6可通过有效抑制Wnt/β-连环蛋白信号通路降低β-连环蛋白的表达来诱导凋亡。
