Dasgupta Pallavi, Kumar Vinay, Krishnaswamy Patnam R, Bhat Navakanta
Centre for Nanoscience and Engineering, Indian Institute of Science Bengaluru India
PathShodh Healthcare Pvt. Ltd. Bengaluru India.
RSC Adv. 2020 Oct 26;10(64):39092-39101. doi: 10.1039/d0ra06470j. eCollection 2020 Oct 21.
Creatinine is a reliable indicator of renal function and degradation of muscular metabolism. Current analytical techniques for its measurement are limited by their cost and requirement of sophisticated instruments. In this work, we report a highly sensitive amperometric biosensor for creatinine by utilizing the one-step selective conversion of creatinine by creatinine deiminase. The novelty of the proposed sensor relies on the measurement of -methylhydantoin produced in the reaction. The sensing chemistry comprises of creatinine deiminase as the receptor for creatinine, cobalt chloride as the electrochemically active recognition element, and methylene blue as the redox mediator. The sensing chemistry is immobilized on the glassy carbon electrode surface by physisorption. We have been able to provide a standalone device that reliably quantifies creatinine in serum and even whole blood, without any sample pre-processing. It is possible to measure creatinine in the clinically relevant range from 0.8 to 4 mg dL with this approach.
肌酐是肾功能和肌肉代谢降解的可靠指标。目前用于其测量的分析技术受到成本和对精密仪器要求的限制。在这项工作中，我们报道了一种通过利用肌酐脱亚氨酶对肌酐的一步选择性转化来检测肌酐的高灵敏度安培生物传感器。所提出的传感器的新颖之处在于对反应中产生的甲基乙内酰脲的测量。传感化学由作为肌酐受体的肌酐脱亚氨酶、作为电化学活性识别元件的氯化钴和作为氧化还原介质的亚甲基蓝组成。传感化学通过物理吸附固定在玻碳电极表面。我们能够提供一种独立的设备，无需任何样品预处理就能可靠地定量血清甚至全血中的肌酐。用这种方法可以在0.8至4mg/dL的临床相关范围内测量肌酐。
