Program in Biotechnology, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand.
Metallurgy and Materials Science Research Institute, Chulalongkorn University, Soi Chula 12, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.
Anal Chim Acta. 2019 Nov 20;1083:110-118. doi: 10.1016/j.aca.2019.07.029. Epub 2019 Jul 17.
A paper-based analytical device (PAD) with an integrated composite electrode for non-enzymatic creatinine sensing was developed. The electrode was produced and optimization was efficiently accomplished using a rapid digital dispensing approach. The electrochemical sensor was fabricated using an HP D300 digital dispenser to deliver a copper oxide and ionic liquid composite onto an electrochemically reduced graphene modified screen-printed carbon electrode (CuO/IL/ERGO/SPCE) on a PAD. The modified electrode was characterized using electrochemical and microscopic techniques. Electrochemical detection of creatinine was performed on the SPCE using amperometry at a constant potential. Under optimized conditions, the paper-based sensor exhibited a linear range of 0.01-2.0 mM (R = 0.99) and the limit of detection was 0.22 μM (S/N = 3, IUPAC definition) for creatinine. The simple fabrication process, low cost, and clinically appropriate creatinine sensitivity make this device applicable for point-of-care use.
一种基于纸张的分析器件（PAD），带有集成的复合电极，用于非酶肌酐感测。该电极通过快速数字分配方法进行了制作和优化。电化学传感器使用 HP D300 数字分配器在 PAD 上将氧化铜和离子液体复合层涂覆在经电化学还原的氧化石墨烯修饰的丝网印刷碳电极（CuO/IL/ERGO/SPCE）上。使用电化学和显微镜技术对修饰电极进行了表征。在 SPCE 上使用恒电位安培法进行肌酐的电化学检测。在优化条件下，基于纸张的传感器对肌酐表现出 0.01-2.0 mM 的线性范围（R = 0.99），肌酐的检测限为 0.22 μM（S/N = 3，IUPAC 定义）。该器件具有简单的制造工艺、低成本和临床适当的肌酐灵敏度，适用于即时护理用途。
