Szczęch Marta, Orsi Davide, Łopuszyńska Natalia, Cristofolini Luigi, Jasiński Krzysztof, Węglarz Władysław P, Albertini Franca, Kereïche Sami, Szczepanowicz Krzysztof
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences Krakow Poland
Department of Mathematical, Physical and Computer Sciences, University of Parma Parma Italy.
RSC Adv. 2020 Dec 8;10(71):43607-43618. doi: 10.1039/d0ra07507h. eCollection 2020 Nov 27.
There are huge demands on multifunctional nanocarriers to be used in nanomedicine. Herein, we present a simple and efficient method for the preparation of multifunctional magnetically responsive polymeric-based nanocarriers optimized for biomedical applications. The hybrid delivery system is composed of drug-loaded polymer nanoparticles (poly(caprolactone), PCL) coated with a multilayer shell of polyglutamic acid (PGA) and superparamagnetic iron oxide nanoparticles (SPIONs), which are known as bio-acceptable components. The PCL nanocarriers with a model anticancer drug (Paclitaxel, PTX) were formed by the spontaneous emulsification solvent evaporation (SESE) method, while the magnetically responsive multilayer shell was formed the layer-by-layer (LbL) method. As a result, we obtained magnetically responsive polycaprolactone nanocarriers (MN-PCL NCs) with an average size of about 120 nm. Using the 9.4 T preclinical magnetic resonance imaging (MRI) scanner we confirmed, that obtained MN-PCL NCs can be successfully used as a MRI-detectable drug delivery system. The magnetic hyperthermia effect of the MN-PCL NCs was demonstrated by applying a 25 mT radio-frequency ( = 429 kHz) alternating magnetic field. We found a Specific Absorption Rate (SAR) of 55 W g. The conducted research fulfills the first step of investigation for biomedical application, which is mandatory for the planning of any and studies.
纳米医学中对多功能纳米载体有巨大需求。在此，我们提出一种简单高效的方法来制备针对生物医学应用优化的多功能磁响应聚合物基纳米载体。这种混合递送系统由负载药物的聚合物纳米颗粒（聚己内酯，PCL）组成，其表面包覆有聚谷氨酸（PGA）和超顺磁性氧化铁纳米颗粒（SPIONs）的多层壳，这些都是生物可接受的成分。含有模型抗癌药物（紫杉醇，PTX）的PCL纳米载体通过自发乳化溶剂蒸发（SESE）法形成，而磁响应多层壳则通过层层（LbL）法形成。结果，我们获得了平均尺寸约为120 nm的磁响应聚己内酯纳米载体（MN - PCL NCs）。使用9.4 T临床前磁共振成像（MRI）扫描仪，我们证实所获得的MN - PCL NCs可成功用作MRI可检测的药物递送系统。通过施加25 mT射频（ = 429 kHz）交变磁场证明了MN - PCL NCs的磁热疗效果。我们发现比吸收率（SAR）为55 W g。所进行的研究完成了生物医学应用研究的第一步，这对于任何和研究的规划都是必不可少的。
