Dassamiour Saliha, Meguellati Selsabil, Lamraoui Hdouda, Bensaad Mohamed Sabri, Sami Rokayya, Alshehry Garsa, Althubaiti Eman Hillal, Al-Meshal Areej Suliman
Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2 Fesdis Batna 05078 Algeria
Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2 Fesdis Batna 05078 Algeria
RSC Adv. 2022 May 4;12(21):13330-13338. doi: 10.1039/d2ra01630c. eCollection 2022 Apr 28.
The date palm ( L.) is one of the most important crops in arid and semi-arid zones. Date fruit occupies a good place in traditional medicine among the Saharan residents, due to its therapeutic virtues; although there may be several therapeutic virtues yet to be discovered. The aim of this study was to investigate the phytochemical and pharmacological properties of the hexanic (EHx), chloroformic (ECh), ethyl acetate (EAc) and aqueous (EAq) extracts of Tanteboucht pulp. The phytochemical characterization and estimation of phenolic compounds were done based on an HPLC-DAD approach. The antioxidant activity was evaluated by a DPPH scavenging effect test. The sensitivity of 7 bacterial strains and to Tanteboucht extracts was tested using the diffusion disc on agar medium method. The membrane stabilization test was used to determine the anti-inflammatory effect of the fruit extracts. Fourteen phenolic compounds were detected in organic extracts and EAc was the richest followed by ECh and finally EHx which was very poor in these molecules. All extracts showed antioxidant, anti-inflammatory and antimicrobial properties which differ in rate. Indeed, ECh had the greatest scavenging effect on DPPH, followed by EAc and then EAq. EAc was the most potent inhibitor of microbial strains. EAc and ECh were more efficient at membrane stabilization followed by EAq and the three extracts had more anti-inflammatory capacity than the positive control acetyl salicylic acid. The obtained considerable activities were significantly correlated with flavonoid and tannin contents in the extracts.
海枣（L.）是干旱和半干旱地区最重要的作物之一。海枣果实因其治疗功效，在撒哈拉居民的传统医学中占据重要地位；尽管可能还有一些治疗功效有待发现。本研究的目的是调查坦特布赫特果肉的正己烷提取物（EHx）、氯仿提取物（ECh）、乙酸乙酯提取物（EAc）和水提取物（EAq）的植物化学和药理特性。基于高效液相色谱 - 二极管阵列检测（HPLC - DAD）方法对酚类化合物进行植物化学表征和定量分析。通过二苯基苦味酰基自由基（DPPH）清除效应试验评估抗氧化活性。采用琼脂平板扩散法测试7种细菌菌株对坦特布赫特提取物的敏感性。使用膜稳定试验来确定果实提取物的抗炎作用。在有机提取物中检测到14种酚类化合物，乙酸乙酯提取物中含量最丰富，其次是氯仿提取物，最后是正己烷提取物，其这些分子含量非常低。所有提取物均表现出抗氧化、抗炎和抗菌特性，但其程度有所不同。实际上，氯仿提取物对DPPH的清除效果最佳，其次是乙酸乙酯提取物，然后是水提取物。乙酸乙酯提取物是最有效的微生物菌株抑制剂。乙酸乙酯提取物和氯仿提取物在膜稳定方面更有效，其次是水提取物，并且这三种提取物的抗炎能力均强于阳性对照乙酰水杨酸。所获得的显著活性与提取物中的黄酮类化合物和单宁含量显著相关。
