Shim Sun-Yup, Lee Ye Eun, Song Hwa Young, Lee Mina
Fish Health Center, Chonnam National University, 50 Daehak-Ro, Yeosu-si, Jeonnam 59626, Korea.
College of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon-si, Jeonnam 57922, Korea.
Antioxidants (Basel). 2020 Mar 21;9(3):258. doi: 10.3390/antiox9030258.
This study evaluated bioactivity-guided fractionation as a means to identify therapeutic phytochemicals from that can attenuate melanogenesis and oxidation. Seven compounds with inhibitory effects on melanin production and tyrosinase (TYR) activity, and ABTS and DPPH radical-scavenging activities, which have not been reported as whitening materials, were isolated from the -butanol fraction from leaves (PAL). Among the seven compounds, -hydroxybenzoic acid β-d-glucosylester (HG), and cimidahurinine (CH) had strong inhibitory effects on melanin production and TYR activity, as well as ABTS and DPPH radical-scavenging activities. Western blot analysis showed that HG and CH suppressed tyrosinase-related protein (TYRP)-1 and TYRP-2 expression. Moreover, HG and CH inhibited reactive oxygen species (ROS) generation in tert-butyl hydroperoxide (-BHP)-treated B16F10 cells. These results suggest that containing active compounds, such as HG and CH, is a potent therapeutic candidate for the development of hypopigmenting agents.
本研究评估了生物活性导向分级分离法，以此作为从[植物名称]中鉴定可减弱黑色素生成和氧化的治疗性植物化学物质的一种手段。从[植物名称]叶的正丁醇馏分（PAL）中分离出七种对黑色素生成和酪氨酸酶（TYR）活性具有抑制作用，且具有ABTS和DPPH自由基清除活性的化合物，这些化合物此前未被报道为美白物质。在这七种化合物中，对羟基苯甲酸β - D - 葡萄糖酯（HG）和环咪唑啉（CH）对黑色素生成和TYR活性以及ABTS和DPPH自由基清除活性具有强烈抑制作用。蛋白质印迹分析表明，HG和CH抑制了酪氨酸酶相关蛋白（TYRP）-1和TYRP - 2的表达。此外，HG和CH抑制了叔丁基过氧化氢（t - BHP）处理的B16F10细胞中活性氧（ROS）的生成。这些结果表明，含有HG和CH等活性化合物的[植物名称]是开发色素减退剂的有力治疗候选物。
