皮升微孔阵列中哺乳动物细胞的一步式DNA扩增。
One step DNA amplification of mammalian cells in picoliter microwell arrays.
作者信息
Liu Wenwen, Li Zhao, Liu Yuanjie, Wei Qingquan, Liu Yong, Ren Lufeng, Wang Chenyu, Yu Yude
机构信息
State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences P. O. Box 912 Beijing 100083 China
出版信息
RSC Adv. 2019 Jan 21;9(5):2865-2869. doi: 10.1039/c8ra06717a. eCollection 2019 Jan 18.
Abstract
We developed a strategy for direct DNA amplification of single cells on a PEG-modified silica chip with 30 600 picoliter-sized microwells. HPV-positive cells in heterogeneous populations were successfully detected with high accuracy sensitivity as high as single copy.
摘要
我们开发了一种在具有30600皮升大小微孔的聚乙二醇修饰硅胶芯片上对单细胞进行直接DNA扩增的策略。在异质群体中，人乳头瘤病毒（HPV）阳性细胞被成功检测到，其灵敏度高达单拷贝水平，准确性高。
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