Hahn Jungwoo, Kim Eunghee, Han Hyebin, Choi Young Jin
Center for Food and Bioconvergence, Seoul National University 1 Gwanakro, Gwanakgu Seoul 151-921 Korea.
Department of Agricultural Biotechnology, Seoul National University 1 Gwanakro, Gwanakgu Seoul 151-921 Korea
RSC Adv. 2020 Aug 24;10(52):31243-31250. doi: 10.1039/d0ra05115b. eCollection 2020 Aug 21.
We have developed a low-cost, portable lab-on-a-valve (LOV) integrated microdevice for the detection of pathogens in primary-diagnosis settings. This system was designed for field-based pathogen detection based on the aggregation of gold nanoparticles induced by a switchable linker. A three-way valve, which has attracted much attention as a functional mesofluidic platform for pressure-driven flow, has been designed as a universal reaction platform that combines the functions of fluid flow and a reaction chamber. In addition, we obtain rapid and enhanced visual signals by the use of a syringe filter to remove gold nano-aggregates (Au NAs). Using this device, Typhimurium down to 10 CFU mL can be visually detected within 30 min by performing a simple operation that requires no complex equipment. This prototype device has great potential for use in the semi-quantitative and qualitative identification of pathogens in on-site primary diagnoses.
我们开发了一种低成本、便携式的阀上实验室（LOV）集成微器件，用于在初级诊断环境中检测病原体。该系统基于可切换连接体诱导的金纳米颗粒聚集，设计用于现场病原体检测。三通阀作为一种用于压力驱动流的功能性介观流体平台备受关注，已被设计为一个通用反应平台，它结合了流体流动和反应室的功能。此外，我们通过使用注射器过滤器去除金纳米聚集体（Au NAs）来获得快速增强的视觉信号。使用该设备，通过执行无需复杂设备的简单操作，在30分钟内即可目视检测到低至10 CFU/mL的鼠伤寒沙门氏菌。这种原型设备在现场初级诊断中对病原体进行半定量和定性鉴定方面具有巨大的应用潜力。
