Wang Binshuai, Song Yimeng, Ge Liyuan, Zhang Shudong, Ma Lulin
Department of Urology, Peking University Third Hospital Beijing 100191 China
RSC Adv. 2019 Mar 22;9(17):9379-9385. doi: 10.1039/c8ra08682f.
In recent years, liquid biopsies, especially for detecting circulating tumor cells (CTCs), have received great attention for cancer diagnosis and treatment monitoring. For clinical diagnosis of prostate cancer (PCa), prostate specific antigen (PSA) has been widely used as a standard method for PCa screening. However, PSA diagnostic efficacy within early-stage PCa patients with a PSA level of 4-10 ng mL is always controversial. Therefore, the development of new methods to assist clinical PSA diagnosis is greatly desired. Herein, we report the fabrication of antibody-modified reduced graphene oxide films, which can be used to efficiently detect CTCs in PCa patients with PSA levels of 4-10 ng mL. The antibody-modified reduced graphene oxide (rGO) films were fabricated by spray coating reduced graphene oxide solution onto a smooth glass slide and then modifying it with anti-epithelial cell adhesion molecules (anti-EpCAMs) and anti-prostate specific membrane antigen (anti-PSMA). The rGO films exhibited an excellent ability to capture CTCs from the blood of PCa patients with PSA levels of 4-10 ng mL and the efficiency could reach 60% (6/10). Our approach for highly efficient detection of CTCs in early-stage PCa patients may provide great potential in assisting clinical cancer diagnosis.
近年来，液体活检，尤其是用于检测循环肿瘤细胞（CTC）的技术，在癌症诊断和治疗监测方面受到了广泛关注。对于前列腺癌（PCa）的临床诊断，前列腺特异性抗原（PSA）已被广泛用作PCa筛查的标准方法。然而，PSA在PSA水平为4-10 ng/mL的早期PCa患者中的诊断效能一直存在争议。因此，迫切需要开发新的方法来辅助临床PSA诊断。在此，我们报道了抗体修饰的还原氧化石墨烯薄膜的制备，该薄膜可用于有效检测PSA水平为4-10 ng/mL的PCa患者中的CTC。抗体修饰的还原氧化石墨烯（rGO）薄膜是通过将还原氧化石墨烯溶液喷涂到光滑的载玻片上，然后用抗上皮细胞粘附分子（抗EpCAMs）和抗前列腺特异性膜抗原（抗PSMA）进行修饰而制备的。rGO薄膜表现出优异的能力，能够从PSA水平为4-10 ng/mL的PCa患者血液中捕获CTC，捕获效率可达60%（6/10）。我们用于高效检测早期PCa患者中CTC的方法可能在辅助临床癌症诊断方面具有巨大潜力。
