Li Dongying, Chen Meigui, Guo Wenmin, Li Pin, Wang Haoyu, Ding Wenhao, Li Mengqi, Xu Yong
Key Laboratory of Hunan Province for Efficient Power System and Intelligent Manufacturing, College of Mechanical and Energy Engineering, Shaoyang University, Shaoyang 422000, China.
ACS Omega. 2022 Mar 31;7(14):12242-12254. doi: 10.1021/acsomega.2c00629. eCollection 2022 Apr 12.
Graphene oxide (GO) and nanohydroxyapatite (nHA) are usually used for improving the strength and bioactivity of polymer scaffolds. However, due to the nano-aggregation effect, these applications often face the problems of uneven dispersion and poor interface bonding. In this work, their hybrids (GO@nHA) were constructed by combining chemical modification and in situ growth methods, realizing the perfect combination of nHA and GO. First, the functionalization of GO was realized through oxidative self-polymerization of dopamine (DA), and the product was denoted GO@DA. Furthermore, the in situ growth of nHA on GO@DA was induced by hydrothermal reactions to prepare GO@nHA hybrids. Then, the obtained hybrid was added to the polymer matrix, and a composite scaffold was prepared through a selective laser sintering process. The results demonstrated that with the addition of GO@DA and GO@nHA, the ultimate strength was increased to 16.8 and 18.6 MPa, respectively, which is 66 and 84% higher than the 10.1 MPa of the polylactic acid (PLA) scaffold. In addition, composite scaffolds exhibited good biomineralization ability in vitro and also promoted the adhesion and proliferation of MG63 cells.
氧化石墨烯（GO）和纳米羟基磷灰石（nHA）通常用于提高聚合物支架的强度和生物活性。然而，由于纳米聚集效应，这些应用常常面临分散不均匀和界面结合不良的问题。在这项工作中，通过化学修饰和原位生长方法构建了它们的杂化物（GO@nHA），实现了nHA与GO的完美结合。首先，通过多巴胺（DA）的氧化自聚合实现了GO的功能化，产物记为GO@DA。此外，通过水热反应诱导nHA在GO@DA上原位生长，制备了GO@nHA杂化物。然后，将所得杂化物添加到聚合物基体中，通过选择性激光烧结工艺制备了复合支架。结果表明，添加GO@DA和GO@nHA后，极限强度分别提高到了16.8和18.6 MPa，比聚乳酸（PLA）支架的10.1 MPa分别高出66%和84%。此外，复合支架在体外表现出良好的生物矿化能力，还促进了MG63细胞的黏附和增殖。
