McFarland J G, Aster R H
Blood. 1987 May;69(5):1425-30.
Four platelet compatibility assays were performed on serum and platelet or lymphocyte samples from 38 closely HLA-matched donor/recipient pairs involved in 55 single-donor platelet transfusions. The 22 patients studied were refractory to transfusions of pooled random-donor platelets. Of the four assays (platelet suspension immunofluorescence, PSIFT; 51Cr release; microlymphocytotoxicity; and a monoclonal anti-IgG assay, MAIA), the MAIA was most predictive of platelet transfusion outcome (predictability, 74% for one-hour posttransfusion platelet recovery and 76% for 24-hour recovery). The only other assay to reach statistical significance was the PSIFT (63% predictability for one-hour posttransfusion recovery). The degree of HLA compatibility between donor and recipient (exact matches v those utilizing cross-reactive associations) was unrelated to the ability of the MAIA to predict transfusion results. The MAIA may be capable of differentiating HLA antibodies, ABO antibodies, and platelet-specific antibodies responsible for failure of HLA-matched and selectively mismatched single-donor platelet transfusions.
对参与55次单供体血小板输注的38对HLA紧密匹配的供体/受体对的血清、血小板或淋巴细胞样本进行了四项血小板相容性检测。所研究的22名患者对混合随机供体血小板输注无效。在四项检测中（血小板悬浮免疫荧光法，PSIFT；51Cr释放法；微量淋巴细胞毒性法；以及单克隆抗IgG检测法，MAIA），MAIA对血小板输注结果的预测性最强（输血后1小时血小板回收率的预测性为74%，24小时回收率的预测性为76%）。唯一达到统计学显著性的另一项检测是PSIFT（输血后1小时回收率的预测性为63%）。供体与受体之间的HLA相容程度（完全匹配与利用交叉反应关联的匹配）与MAIA预测输血结果的能力无关。MAIA或许能够区分导致HLA匹配和选择性错配的单供体血小板输注失败的HLA抗体、ABO抗体和血小板特异性抗体。
