Li Xizhe, Fu Tao, Li Bingyu, Yan Peng, Wu Yayan
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University 710049 Xi'an PR China
RSC Adv. 2019 Apr 30;9(23):13275-13282. doi: 10.1039/c9ra02079a. eCollection 2019 Apr 25.
Developing silver nanomaterials with efficient antimicrobial properties is of importance for combating bacteria. Here, we report ultrasmall riboflavin-protected silver nanoclusters (RF@AgNCs) that can effectively kill or suppress the growth of Gram-positive , Gram-negative , and fungi . Riboflavin (RF) with intrinsic biocompatibility was used as a surface ligand to synthesize silver nanoclusters. TEM revealed that the synthesized RF@AgNCs were ultrasmall (2.4 ± 1.2 nm), spherical and well-dispersed. Antibacterial activity tests showed that RF@AgNCs possessed superior antibacterial efficacy in comparison with RF, AgNPs and mixed RF and AgNPs (RF + AgNPs). The mechanisms of antibacterial activity of RF@AgNCs were studied by fluorescence microscopy-based Live/Dead cell staining assays and ROS measurement. And the results illustrated that the integrity of the bacteria membrane was disrupted and intracellular high level ROS generation was induced by RF@AgNCs. The cytotoxic activities were also assessed and RF@AgNCs were found to be non-toxic to human red blood cells and mammalian cells. With the highly efficient antibacterial activity and acceptable biocompatibility, RF@AgNCs hold great promise in biomedical applications as well as in water sterilization and the textile industry.
开发具有高效抗菌性能的银纳米材料对于对抗细菌至关重要。在此，我们报道了超小的核黄素保护的银纳米团簇（RF@AgNCs），其能够有效杀灭或抑制革兰氏阳性菌、革兰氏阴性菌和真菌的生长。具有内在生物相容性的核黄素（RF）被用作表面配体来合成银纳米团簇。透射电子显微镜显示合成的RF@AgNCs超小（2.4±1.2纳米），呈球形且分散良好。抗菌活性测试表明，与RF、AgNPs以及混合的RF和AgNPs（RF + AgNPs）相比，RF@AgNCs具有卓越的抗菌效果。通过基于荧光显微镜的活/死细胞染色分析和活性氧测量研究了RF@AgNCs的抗菌活性机制。结果表明，RF@AgNCs破坏了细菌膜的完整性并诱导细胞内产生高水平的活性氧。还评估了细胞毒性活性，发现RF@AgNCs对人类红细胞和哺乳动物细胞无毒。凭借高效的抗菌活性和可接受的生物相容性，RF@AgNCs在生物医学应用以及水消毒和纺织工业中具有广阔的前景。
