Zheng Yan-Hui, Li Wen-Hong, Chen Peng, Zhou Ying, Lu Wenbo, Ma Zi-Chuan
College of Chemistry and Material Science, Hebei Normal University Shijiazhuang 050024 China
Shijiazhuang Vocational College of Finance & Economics Shijiazhuang 050061 China.
RSC Adv. 2020 Nov 4;10(66):40136-40141. doi: 10.1039/d0ra07573f. eCollection 2020 Nov 2.
In this work, a method for the determination of berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology is established. Berberine is the main extract of Rhizoma coptidis, a medicinal material, which causes an envelope reaction with β-cyclodextrin to generate fluorescence sensitization. In the environment of its own aqueous extract, with 0.0065 mol L of β-cyclodextrin, a fluorescence excitation wavelength ( ) of 345 nm and an emission wavelength ( ) of 540 nm were selected to avoid interference from other distractors. The fluorescent sensor for the detection of berberine exhibits a low limit of detection (3.59 × 10 mol L) and a wide linear range from 2.7 × 10 mol L to 2.7 × 10 mol L. Our sensor can be also used to detect berberine in real medicinal materials. The content of berberine in Rhizoma coptidis medicinal material was found to be 7.60% using this method with an average recovery rate of 99.5%. The result obtained by thin-layer chromatography with fluorescence detection was 7.61%, which is consistent with the result from the β-cyclodextrin sensitized fluorescence method. This method is simple and environmentally friendly with high sensitivity and good selectivity and gives reliable results, which is promising for practical application.
本研究建立了一种利用β-环糊精敏化荧光技术测定黄连中黄连素的方法。黄连素是药材黄连的主要提取物，它与β-环糊精发生包络反应产生荧光增敏。在其自身水提取物的环境中，加入0.0065 mol/L的β-环糊精，选择荧光激发波长（ ）为345 nm，发射波长（ ）为540 nm，以避免其他干扰物的干扰。用于检测黄连素的荧光传感器具有低检测限（3.59×10 mol/L）和宽线性范围，从2.7×10 mol/L到2.7×10 mol/L。我们的传感器还可用于检测实际药材中的黄连素。用该方法测得黄连药材中黄连素的含量为7.60%，平均回收率为99.5%。荧光检测薄层色谱法得到的结果为7.61%，与β-环糊精敏化荧光法的结果一致。该方法简单、环保，具有高灵敏度和良好的选择性，结果可靠，具有实际应用前景。
