Gong Jin, Liu Chang, Jiao Xiaojie, He Song, Zhao Liancheng, Zeng Xianshun
Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology Tianjin 300384 China
School of Materials Science and Engineering, Harbin Institute of Technology Harbin 150001 China.
RSC Adv. 2020 Aug 10;10(49):29536-29542. doi: 10.1039/d0ra04373g. eCollection 2020 Aug 5.
In this paper, we report a novel near-infrared (NIR) mitochondrion-targeted fluorescent probe, RQS, with an improved Stokes shift (96 nm) for the specific detection of mitochondrial mercury ion (Hg) because mitochondrion is one of the main targeted organelles of Hg. For the preparation of the probe, a novel asymmetrical fluorescent xanthene dye RQ was first synthesized by tuning the donor-acceptor-donor (D-A-D) character of the rhodamine skeleton, and then the probe RQS was constructed by the mechanism of mercury-promoted ring-opening reaction. As expected, RQS could be used for the specific detection of Hg with high selectivity, high sensitivity, and a detection limit down to the nanomolar range (2 nM). Importantly, RQS is capable of specifically distributing in mitochondria, and thus detect Hg in real-time and provided a potential tool for studying the cytotoxic mechanisms of Hg.
在本文中，我们报道了一种新型近红外（NIR）线粒体靶向荧光探针RQS，其斯托克斯位移得到了改善（96 nm），用于特异性检测线粒体汞离子（Hg），因为线粒体是Hg的主要靶向细胞器之一。为了制备该探针，首先通过调节罗丹明骨架的供体-受体-供体（D-A-D）特性合成了一种新型不对称荧光呫吨染料RQ，然后通过汞促进的开环反应机制构建了探针RQS。正如预期的那样，RQS可用于高选择性、高灵敏度地特异性检测Hg，检测限低至纳摩尔范围（2 nM）。重要的是，RQS能够特异性地分布在线粒体中，从而实时检测Hg，并为研究Hg的细胞毒性机制提供了一种潜在工具。
