大鼠脊髓副交感神经节前区域中含蛋氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8样免疫反应性神经元的免疫组织化学研究

An immunohistochemical study of methionine-enkephalin-Arg6-Gly7-Leu8-like immunoreactivity-containing neurons in the parasympathetic preganglionic regions of the rat spinal cord.

作者信息

Shimosegawa T, Koizumi M, Toyota T, Goto Y, Yanaihara C, Yanaihara N

出版信息

Brain Res. 1987 Mar 17;406(1-2):341-7. doi: 10.1016/0006-8993(87)90804-3.

Abstract

The localization of the methionine-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu)-like immunoreactivity-containing neurons in the rat lumbosacral spinal cord was immunohistochemically examined by an antiserum very specific to Met-Enk-Arg-Gly-Leu. The immunoreactive neurons occupied the positions corresponding to the parasympathetic preganglionic nuclei determined by the previous horseradish peroxidase (HRP)-tracing experiments. The present study suggests that the parasympathetic preganglionic neurons in the rat lumbosacral spinal cord produce preproenkephalin A and its related peptides.

摘要

用一种对甲硫氨酸脑啡肽-精氨酸6-甘氨酸7-亮氨酸8（Met-Enk-Arg-Gly-Leu）具有高度特异性的抗血清，通过免疫组织化学方法检测大鼠腰骶脊髓中含Met-Enk-Arg-Gly-Leu样免疫反应性的神经元的定位。这些免疫反应性神经元占据了先前通过辣根过氧化物酶（HRP）追踪实验确定的副交感神经节前核相对应的位置。本研究表明，大鼠腰骶脊髓中的副交感神经节前神经元产生前脑啡肽原A及其相关肽。