George J R, Pine L, Reeves M W, Harrell W K
J Clin Microbiol. 1980 Mar;11(3):286-91. doi: 10.1128/jcm.11.3.286-291.1980.
The amino acids required for growth and as energy sources by 10 strains of Legionella pneumophila were determined by using a chemically defined medium. All strains required arginine, cysteine, isoleucine, leucine, threonine, valine, methionine, and phenylalanine or tyrosine. Most strains (7 of 10) required serine, and two strains had to be supplied proline before growth could be established. All 10 strains used serine and, to a lesser extent, threonine as the sole sources of carbon and energy. The Y serine calculated was 94.9 +/- 8.5 g (dry weight) of cells/mol of serine. Assuming that the value of Y adenosine 5'-triphosphate is 10.5, these results indicate that oxidative catabolism of 1 mol of serine yielded approximately 9 mol of adenosine 5'-triphosphate. This high yield suggests that although serine was the major source of carbon, other amino acids may also be metabolized.
通过使用化学成分确定的培养基，测定了10株嗜肺军团菌生长所需以及作为能量来源的氨基酸。所有菌株都需要精氨酸、半胱氨酸、异亮氨酸、亮氨酸、苏氨酸、缬氨酸、甲硫氨酸和苯丙氨酸或酪氨酸。大多数菌株（10株中的7株）需要丝氨酸，有两株在能够生长之前必须供应脯氨酸。所有10株菌株都利用丝氨酸，并且在较小程度上利用苏氨酸作为唯一的碳源和能量来源。计算得出的Y丝氨酸为94.9±8.5克（干重）细胞/摩尔丝氨酸。假设Y三磷酸腺苷的值为10.5，这些结果表明1摩尔丝氨酸的氧化分解代谢产生约9摩尔三磷酸腺苷。这种高产率表明，虽然丝氨酸是主要的碳源，但其他氨基酸也可能被代谢。
