利用异源基因表达研究真菌生物合成途径：构巢曲霉作为异源宿主。
Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Aspergillus nidulans as a Heterologous Host.
机构信息
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA.
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2022;2489:41-52. doi: 10.1007/978-1-0716-2273-5_3.
Abstract
Fungal natural products encompass an important source of pharmaceutically relevant molecules. Heterologous expression of biosynthetic pathways in chassis strains enables the discovery of new secondary metabolites and characterization of pathway enzymes. In our laboratory, biosynthetic genes in a clustered pathway have been refactored in engineered heterologous hosts such as Aspergillus nidulans. Here we describe the assembly of heterologous expression vectors, transformation into A. nidulans, and detection of new compounds in the transformant strains.
摘要
真菌天然产物是具有重要药用价值的分子的重要来源。在底盘菌株中异源表达生物合成途径可以发现新的次级代谢产物并对途径酶进行特征分析。在我们的实验室中，已对工程异源宿主（如构巢曲霉）中簇状途径的生物合成基因进行了重构。本文描述了异源表达载体的构建、转化到构巢曲霉中，以及在转化株中检测新化合物。
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