Department of Medicine, School of Medicine and Public Health, University of Wisconsin Madison, Madison, WI, 53706, USA.
J Ind Microbiol Biotechnol. 2020 Dec;47(12):1109-1116. doi: 10.1007/s10295-020-02328-x. Epub 2020 Nov 18.
Activation of silent biosynthetic gene clusters in fungi remains a challenge for discovery of new useful natural products. In this work, we identify a group of silent secondary metabolite gene clusters in Aspergillus nidulans that are induced by rice (Oryza sativa). Using reverse phase HPLC purification on extracts of rice, we identified the plant hormone gibberellic acid as one compound present in rice extracts that induced these silent genes. Additionally, select secondary metabolite (SM) genes activated by rice were tested for responses to several plant hormones which produced distinctly different transcriptomic profiles in A. nidulans. These observations support the idea that phytohormones play an important role in regulating fungal SM biosynthesis while additionally serving as a source of natural product chemical libraries to screen for useful compounds.
真菌中沉默生物合成基因簇的激活仍然是发现新的有用天然产物的一个挑战。在这项工作中，我们在构巢曲霉中鉴定了一组沉默的次级代谢物基因簇，这些基因簇受水稻（Oryza sativa）诱导。通过对水稻提取物进行反相 HPLC 纯化，我们鉴定出植物激素赤霉素是水稻提取物中诱导这些沉默基因的一种化合物。此外，还测试了由水稻激活的选择次级代谢物（SM）基因对几种植物激素的反应，这些基因在构巢曲霉中产生了明显不同的转录组图谱。这些观察结果支持了这样一种观点，即植物激素在调节真菌 SM 生物合成中起着重要作用，同时还可以作为筛选有用化合物的天然产物化学文库的来源。
