School of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng, 224051, People's Republic of China.
Arch Microbiol. 2022 May 7;204(6):304. doi: 10.1007/s00203-022-02903-z.
Phage-derived therapies are promising treatments in the fight against bacterial diseases as an alternative strategy nowadays. Species of Aeromonas veronii is an important pathogen causing freshwater fish diseases, the findings on genomic sequences of their bacteriophages are limited. In this work, a lytic bacteriophage capable of specifically infecting A. veronii strain AEv1810 was characterized at the gene level and was designated as pAEv1810. Transmission electron microscopic observation revealed that pAEv1810 belongs to the family of Myoviridae. The genome of phage pAEv1810 consists of 235,066 bp with 38.4% G + C content. Twenty-three of 249 putative proteins encoded by this phage have known functions, and four tRNA genes were found in phage genome. Phylogenetic analyses of RNA polymerase and Terminase large subunit revealed that phage pAEv1810 is closing to those phages classified to the genus Petsuvirus.
噬菌体衍生疗法作为一种替代策略，在对抗细菌性疾病方面具有广阔的应用前景。维氏气单胞菌是一种重要的淡水鱼类病原菌，目前对其噬菌体的基因组序列研究有限。本研究对一株能特异性感染维氏气单胞菌 AEv1810 株的裂解性噬菌体进行了基因水平的鉴定，并将其命名为 pAEv1810。透射电子显微镜观察表明，pAEv1810 属于肌尾噬菌体科。噬菌体 pAEv1810 的基因组由 235066bp 组成，GC 含量为 38.4%。该噬菌体编码的 249 个推定蛋白中有 23 个具有已知功能，噬菌体基因组中还发现了 4 个 tRNA 基因。RNA 聚合酶和终止酶大亚基的系统发育分析表明，噬菌体 pAEv1810 与属于 Petsuvirus 属的噬菌体关系密切。
