Fisheries College, Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Jimei University, Xiamen, Fujian, China.
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, China.
Virus Res. 2023 Oct 15;336:199193. doi: 10.1016/j.virusres.2023.199193. Epub 2023 Aug 29.
Aeromonas is one of the most serious pathogens in freshwater aquaculture. Overuse of antibiotics for the treatment of fish diseases has led to the frequent occurrence of drug-resistant strains. Phage therapy is an alternative approach to overcoming the multi-drug resistance associated with antibiotics. In this study, a novel phage phiA034 targeting the host A. veronii A034 was isolated. The phage could infect 14 strains of 4 species Aeromonas. The phage phiA034 displayed head-tail structure with an icosahedral head in the TEM image. At the optimal MOI of 1, it had a latent period of nearly 20 minutes and a burst size of 286 PFU/cell. Besides, the phage phiA034 exhibited high tolerance to a wide range of temperature (30-70 °C) and acid-base (pH 6.0-10.0). The whole genome of phage phiA034 was sequenced with a size of 61,443 bp and annotated with 82 ORFs, mainly related to structure, DNA replication, and lysis. Based on the analysis and comparison of the genomes and proteomes, phage phiA034 could be classified as a novel species of an existing genus Duplodnaviria Heunggongvirae, Uroviricota, Caudoviricetes, Casjensviridae, Sharonstreetvirus. These findings have expanded the species bank and genomes library of bacterial virus and will promote the application of phage therapy in Aeromonas disease.
气单胞菌是淡水水产养殖中最严重的病原体之一。为治疗鱼类疾病而过度使用抗生素导致耐药菌株频繁出现。噬菌体治疗是克服抗生素相关多药耐药性的一种替代方法。在本研究中，分离到一种针对宿主 A. veronii A034 的新型噬菌体 phiA034。该噬菌体可以感染 4 种气单胞菌的 14 个菌株。噬菌体 phiA034 在 TEM 图像中显示出头尾结构，具有二十面体头部。在最佳 MOI 为 1 时，潜伏期接近 20 分钟，爆发量为 286 PFU/细胞。此外，噬菌体 phiA034 对广泛的温度（30-70°C）和酸碱（pH 6.0-10.0）具有很高的耐受性。噬菌体 phiA034 的全基因组测序大小为 61,443 bp，注释了 82 个 ORFs，主要与结构、DNA 复制和裂解有关。基于基因组和蛋白质组的分析和比较，噬菌体 phiA034 可归类为现有属 Duplodnaviria Heunggongvirae、Uroviricota、Caudoviricetes、Casjensviridae、Sharonstreetvirus 的新型种。这些发现扩展了细菌病毒的物种库和基因组文库，并将促进噬菌体治疗在气单胞菌病中的应用。
