School of Biosciences, Mahatma Gandhi University, PD Hills (PO),, Kottayam, Kerala, 686 560, India.
Curr Microbiol. 2022 May 7;79(6):185. doi: 10.1007/s00284-022-02850-4.
The present study has been focused to evaluate the effect of chitosan nanoparticles (CNPs) and gold nanoparticles (AuNPs) on the phytohormone production by rhizospheric Pseudomonas aeruginosa. The gold nanoparticles were synthesized biologically and characterized by UV-Visible spectrophotometry, transmission electron microscopy, and X-ray diffraction analysis. The indole-3-acetic acid (IAA) production by P. aeruginosa supplemented with CNPs and AuNPs was quantified by using Salkowski's method and confirmed by high-performance liquid chromatography (HPLC) analysis. This revealed the effect of 5 mg/mL CNPs and 100 µg/mL AuNPs to enhance the IAA production by P. aeruginosa. By Salkowski's method, 07.16 ± 0.28 and 09.56 ± 0.28 µg/mL of IAA could be detected in the samples prepared from P. aeruginosa supplemented with 5 mg/mL CNPs and 100 µg/mL AuNPs, respectively. HPLC analysis also confirmed the production of IAA by P. aeruginosa. The CNPs and AuNPs-supplemented P. aeruginosa was also found to have enhancement effect on the shoot length (25.25 ± 0.85 cm and 26.57 ± 0.73 cm) and fresh weight (0.94 ± 0.09 g and 0.96 ± 0.09 g) of Vigna unguiculata plants, which highlight the significance of the study and the agricultural promises of nanomaterials-supplemented rhizobacteria.
本研究旨在评估壳聚糖纳米粒子（CNPs）和金纳米粒子（AuNPs）对根际铜绿假单胞菌植物激素产生的影响。金纳米粒子通过紫外可见分光光度法、透射电子显微镜和 X 射线衍射分析进行了生物合成和表征。通过 Salkowski 法和高效液相色谱（HPLC）分析定量了添加 CNPs 和 AuNPs 的铜绿假单胞菌产生的吲哚-3-乙酸（IAA）。这表明 5mg/mL 的 CNPs 和 100μg/mL 的 AuNPs 可增强铜绿假单胞菌的 IAA 产生。通过 Salkowski 法，在添加 5mg/mL CNPs 和 100μg/mL AuNPs 的铜绿假单胞菌样品中分别检测到 07.16±0.28 和 09.56±0.28μg/mL 的 IAA。HPLC 分析也证实了铜绿假单胞菌产生了 IAA。添加 CNPs 和 AuNPs 的铜绿假单胞菌还对豇豆植株的茎长（25.25±0.85cm 和 26.57±0.73cm）和鲜重（0.94±0.09g 和 0.96±0.09g）有增强作用，这突出了该研究的意义和纳米材料补充根际细菌的农业前景。
