Chitosan nanoparticles loaded with thiamine stimulate growth and enhances protection against wilt disease in Chickpea.

Nanoencapsulation is considered as one of the unique technique for increasing the bioavailability, solubility and retention time of bioactive compounds. In this study, thiamine was incorporated into the chitosan nanoparticles and characterized through FTIR, DLS, SEM, TEM and XRD analyses. Zeta potential of the synthesized nanoparticles was found to be 37.7 mV. The encapsulation efficiency of chitosan nanoparticle was 90 ± 3%. Application of thiamine loaded chitosan nanoparticle enhanced seed germination and growth of chickpea seedlings when compared to untreated control seeds. Treated seedlings showed enhanced production of indole acetic acid (IAA). Foliar application of synthesized nanoparticle induced defense enzymes in leaves and roots of chickpea plants. Decreased cell death in the chickpea roots of treated plants was observed when compared to control under green house condition. These results showed that the thiamine loaded chitosan nanoparticle can be used as a growth stimulator as well as a defense activator in chickpea.