Department of Botany, Bharathidasan University, Tiruchirappalli 620024, India.
Department of Botany, Bharathidasan University, Tiruchirappalli 620024, India.
Carbohydr Polym. 2019 May 15;212:169-177. doi: 10.1016/j.carbpol.2019.02.037. Epub 2019 Feb 13.
Nanoencapsulation is considered as one of the unique technique for increasing the bioavailability, solubility and retention time of bioactive compounds. In this study, thiamine was incorporated into the chitosan nanoparticles and characterized through FTIR, DLS, SEM, TEM and XRD analyses. Zeta potential of the synthesized nanoparticles was found to be 37.7 mV. The encapsulation efficiency of chitosan nanoparticle was 90 ± 3%. Application of thiamine loaded chitosan nanoparticle enhanced seed germination and growth of chickpea seedlings when compared to untreated control seeds. Treated seedlings showed enhanced production of indole acetic acid (IAA). Foliar application of synthesized nanoparticle induced defense enzymes in leaves and roots of chickpea plants. Decreased cell death in the chickpea roots of treated plants was observed when compared to control under green house condition. These results showed that the thiamine loaded chitosan nanoparticle can be used as a growth stimulator as well as a defense activator in chickpea.
纳米封装被认为是提高生物活性化合物生物利用度、溶解度和滞留时间的独特技术之一。在这项研究中，将硫胺素掺入壳聚糖纳米粒子中，并通过傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和 X 射线衍射（XRD）分析进行了表征。合成纳米粒子的 Zeta 电位为 37.7 mV。壳聚糖纳米粒子的包封效率为 90±3%。与未处理的对照种子相比，负载硫胺素的壳聚糖纳米粒子的应用增强了鹰嘴豆种子的萌发和生长。处理过的幼苗表现出吲哚乙酸（IAA）产量的提高。合成纳米粒子的叶面施用于鹰嘴豆植物的叶片和根部诱导防御酶。与温室条件下的对照相比，处理过的植物的鹰嘴豆根部的细胞死亡减少。这些结果表明，负载硫胺素的壳聚糖纳米粒子可用作鹰嘴豆的生长刺激剂和防御激活剂。
