Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Nutrients. 2023 Feb 15;15(4):974. doi: 10.3390/nu15040974.
Increasing evidence suggests that royal jelly (RJ) has exceptional biological properties, and that major royal jelly proteins (MRJPs) are the key active factors in RJ. The objective of this study was to compare the difference in the protein content between RJ and MRJPs using non-labeled, quantitative proteomics technology, and to investigate the adjustment features and mechanisms of MRJPs on murine immune functions and the composition of intestinal flora in cyclophosphamide-treated mice. Results showed that, during the process of extracting MRJPs, the ratio of the protein types in the main protein and other proteins decreased significantly, except for MRJP1 and MRJP7, which demonstrated that an enriching effect of MRJP1 and MRJP7 was present during the extraction process. Cyclophosphamide-induced mice were orally administered MRJPs. Results showed that the middle-dose group, which received 0.25 g/(kg·bw) of royal jelly main protein, demonstrated a clear impact on the development of the spleen and liver, the quantity of peripheral blood leukocytes, immunoglobulin content, immune factor level, and the proliferation ability of spleen lymphocytes. A 16S rRNA high-throughput sequencing technology analysis showed that MRJPs could improve the component and richness of intestinal flora and raise the immunity of mice. The above-mentioned results indicated that the application of MRJPs is very likely to have an advantage effect on murine immune functions.
越来越多的证据表明，蜂王浆（RJ）具有特殊的生物学特性，而主要蜂王浆蛋白（MRJPs）是 RJ 的关键活性因子。本研究旨在利用非标记定量蛋白质组学技术比较 RJ 和 MRJPs 之间的蛋白质含量差异，并研究 MRJPs 对环磷酰胺处理小鼠免疫功能和肠道菌群组成的调节作用和机制。结果表明，在提取 MRJPs 的过程中，除了 MRJP1 和 MRJP7 外，主要蛋白质和其他蛋白质的蛋白质类型比例显著下降，这表明在提取过程中存在 MRJP1 和 MRJP7 的富集作用。将 MRJPs 经口给予环磷酰胺诱导的小鼠。结果表明，中剂量组（0.25 g/（kg·bw）蜂王浆主蛋白）对脾脏和肝脏的发育、外周血白细胞数量、免疫球蛋白含量、免疫因子水平和脾淋巴细胞的增殖能力均有明显影响。16S rRNA 高通量测序技术分析表明，MRJPs 可以改善肠道菌群的组成和丰富度，提高小鼠的免疫力。上述结果表明，MRJPs 的应用很可能对小鼠的免疫功能具有优势作用。
