Yan Shuo, Li Mingjian, Jiang Qinhong, Li Mingshan, Hu Mengfan, Shi Xueyan, Liang Pei, Yin Meizhen, Gao Xiwu, Shen Jie, Zhang Lei
College of Plant Protection, China Agricultural University, 100193 Beijing, PR China.
College of Plant Protection, China Agricultural University, 100193 Beijing, PR China.
J Adv Res. 2025 Jan;67:93-104. doi: 10.1016/j.jare.2024.01.031. Epub 2024 Jan 28.
Unscientific application of insecticides has led to severe resistance of pests to almost all classes of insecticides. Enhanced detoxification is the most common mechanism for this kind of resistance.
Fall armyworm (FAW) has developed insecticide resistance, which is often linked to the overexpression of detoxification genes. Herein, a multicomponent nano-pesticide is designed to increase its broad-spectrum susceptibility toward insecticides.
Regulatory function of nuclear factor erythroid 2-related factor 2 (Nrf2) in detoxification was confirmed using transcriptome sequencing, quantitative real-time PCR and enzyme activity measurement. A star polycation (SPc) was adopted to construct the pesticide/SPc/complex, whose self-assembly mechanism and characterization were examined using isothermal titration calorimetry, dynamic light scattering and transmission electron microscope. The delivery efficiency of SPc-loaded dsRNA was examined in vitro and in vivo using fluorescent tracer technique. A multicomponent nano-pesticide was created through the integration of bacterial expression system and nano-delivery system, and its bioactivity was tested in laboratory and field.
We confirmed the crucial role of Nrf2 in regulating the detoxification in FAW, and silencing Nrf2 could decrease detoxification gene expression and increase insecticide susceptibility. We then applied the SPc to self-assemble a nanoplatform for delivering Nrf2 double-stranded RNA (dsRNA) and pesticide simultaneously. Nano-sized pesticide/SPc/dsRNA complex exhibited high delivery efficiency in vitro and in vivo. Excitingly, the insecticidal activities of pesticide/SPc/dsNrf2 complexes were remarkably improved with the normalized synergistic ratios of 5.43-6.25 for chlorantraniliprole, 4.45-15.00 for emamectin benzoate, and 6.75-15.00 for spinetoram. Finally, we developed a multicomponent nano-pesticide (pesticide/SPc/dsNrf2 complex) using a bacterial expression system and nano-delivery system. This approach exhibited excellent leaf protection and pest control efficacy.
The integration between the pesticide nanometerization and insecticide susceptibility improvement offers a promising strategy to increase insecticidal activity. Our study provides a revolutionary and universal strategy to increase insecticidal activity and decease application doses.
杀虫剂的不科学使用已导致害虫对几乎所有种类的杀虫剂产生严重抗性。解毒作用增强是这种抗性最常见的机制。
草地贪夜蛾已产生抗药性，这通常与解毒基因的过度表达有关。在此，设计了一种多组分纳米农药，以提高其对杀虫剂的广谱敏感性。
利用转录组测序、定量实时聚合酶链反应和酶活性测定，证实了核因子红细胞2相关因子2（Nrf2）在解毒过程中的调节功能。采用星型聚阳离子（SPc）构建农药/SPc/复合物，通过等温滴定量热法、动态光散射和透射电子显微镜研究其自组装机制和特性。利用荧光示踪技术在体外和体内检测了负载SPc的双链RNA（dsRNA）的递送效率。通过整合细菌表达系统和纳米递送系统制备了一种多组分纳米农药，并在实验室和田间测试了其生物活性。
我们证实了Nrf2在调节草地贪夜蛾解毒过程中的关键作用，沉默Nrf2可降低解毒基因表达并提高对杀虫剂的敏感性。然后，我们应用SPc自组装一个纳米平台，用于同时递送Nrf2双链RNA（dsRNA）和农药。纳米级农药/SPc/dsRNA复合物在体外和体内均表现出高递送效率。令人兴奋的是，农药/SPc/dsNrf2复合物的杀虫活性显著提高，氯虫苯甲酰胺的标准化协同比为5.43 - 6.25，甲氨基阿维菌素苯甲酸盐为4.45 - 15.00，多杀霉素为6.75 - 15.00。最后，我们利用细菌表达系统和纳米递送系统开发了一种多组分纳米农药（农药/SPc/dsNrf2复合物）。这种方法表现出优异的叶片保护和害虫防治效果。
农药纳米化与提高杀虫剂敏感性的结合为提高杀虫活性提供了一种有前景的策略。我们的研究提供了一种革命性的通用策略，以提高杀虫活性并减少施用量。
