Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Microbiology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
J Equine Vet Sci. 2022 Jul;114:104004. doi: 10.1016/j.jevs.2022.104004. Epub 2022 May 6.
Antimicrobial administration can lead to imbalances of gastrointestinal microbiota, called dysbiosis. Dysbiosis sometimes results in diarrhea and enteritis in horses. Fecal microbiota transplantation (FMT) is used to treat affected horses, but whether it is effective as a prophylactic approach for dysbiosis in horses receiving antimicrobials remains unknown. The aim of this study was to assess the efficacy of simultaneous FMT against metronidazole-induced dysbiosis in horses. Changes in the ratios of bacterial families, determined by metagenomic analysis, were similar between the metronidazole-treated group and the simultaneous metronidazole- and FMT-treated group, notably in the Clostridiaceae, Ruminococcaceae, and Enterobacteriaceae. Differences in fecal bacterial compositions were due mainly to metronidazole administration (P = .0003), but not to FMT (P = .3136). Simultaneous FMT at 500 g of donor feces in 1 L of suspension once a day did not inhibit metronidazole-induced dysbiosis. The results show that the FMT protocol needs to be improved to prevent metronidazole-induced gut dysbiosis in horses.
抗菌药物的应用会导致胃肠道微生物群落失衡，称为菌群失调。菌群失调有时会导致马出现腹泻和肠炎。粪便微生物群移植（FMT）用于治疗受影响的马匹，但在接受抗菌药物的马匹中，作为预防菌群失调的方法，FMT 是否有效尚不清楚。本研究旨在评估同时进行 FMT 对甲硝唑诱导的马匹菌群失调的疗效。通过宏基因组分析确定的细菌家族比例的变化在甲硝唑治疗组和同时接受甲硝唑和 FMT 治疗组之间相似，尤其是在拟杆菌科、瘤胃球菌科和肠杆菌科。粪便细菌组成的差异主要归因于甲硝唑的给药（P =.0003），而不是 FMT（P =.3136）。每天一次在 1 升悬浮液中使用 500 克供体粪便进行同时 FMT 并不能抑制甲硝唑诱导的菌群失调。结果表明，需要改进 FMT 方案以预防马的甲硝唑诱导的肠道菌群失调。
