Jiangxi Key Laboratory of Nanobiomaterials & Institute of Advanced Materials, East China Jiaotong University, Nanchang 330000, China.
Department of Burn, The First Affiliated Hospital of Nanchang University, Nanchang 330013, China.
Biomater Adv. 2022 Mar;134:112591. doi: 10.1016/j.msec.2021.112591. Epub 2021 Dec 5.
Improving antibacterial performance is one of the prerequisites for the clinical application of bacterial cellulose (BC)-based dressings. In this study, a novel copper (Cu) ion loaded BC-based antibacterial wound dressing was prepared via codeposition of polydopamine (PDA) and copper ion. The scanning electron microscope (SEM) results showed that the copper ion/PDA coating was more uniform than the PDA coating, and the 3D nanopore structure of BC was retained in Cu@PBC. Cu ions were immobilized by forming a chelate with PDA. The thermal stability and mechanical properties of the Cu@PBC dressing decreased with the addition of copper ions. Cu@PBC-2 film with a certain amount of copper sulfate used (10 nM) exhibited favorable antibacterial properties against both S. aureus and E. coli without obvious cytotoxicity. The results of the in vivo study also demonstrated that the Cu@PBC-2 film can eliminate S. aureus infections and inflammatory response, promote collagen deposition, capillary angiogenesis, hair follicle growth and wound healing. These results suggest that the Cu@PBC-2 film has prospective application as a wound dressing.
提高抗菌性能是将基于细菌纤维素（BC）的敷料临床应用的前提条件之一。在本研究中，通过聚多巴胺（PDA）和铜离子共沉积制备了一种新型的载铜（Cu）离子的基于 BC 的抗菌伤口敷料。扫描电子显微镜（SEM）结果表明，铜离子/PDA 涂层比 PDA 涂层更均匀，并且在 Cu@PBC 中保留了 BC 的 3D 纳米孔结构。Cu 离子通过与 PDA 形成螯合物而固定。Cu@PBC 敷料的热稳定性和机械性能随铜离子的加入而降低。Cu@PBC-2 薄膜使用一定量的硫酸铜（10 nM）对金黄色葡萄球菌和大肠杆菌均表现出良好的抗菌性能，且无明显细胞毒性。体内研究结果也表明，Cu@PBC-2 薄膜可以消除金黄色葡萄球菌感染和炎症反应，促进胶原蛋白沉积、毛细血管生成、毛囊生长和伤口愈合。这些结果表明，Cu@PBC-2 薄膜有望作为一种伤口敷料应用。
