School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Department of Medical Information Engineering, Kangda College of Nanjing Medical University, Lianyungang 222000, China.
Carbohydr Polym. 2023 Jun 1;309:120681. doi: 10.1016/j.carbpol.2023.120681. Epub 2023 Feb 8.
Bacterial overgrowth in injured wounds causes wound infection and excessive inflammation, leading to delayed wound healing. Successful treatment of delayed infected wound healing demands dressings, which can inhibit bacterial growth and inflammation and simultaneously induce vascularization, collagen deposition, and re-epithelialization of wounds. In this study, bacterial cellulose (BC) deposited with Cu-loaded phase-transited lysozyme (PTL) nanofilm (BC/PTL/Cu) was prepared for healing infected wounds. The results confirm that PTL were successfully self-assembled on BC matrix, and Cu were loaded into PTL through electrostatic coordination. The tensile strength and the elongation at break of the membranes were not significantly changed after modification with PTL and Cu. Compared with BC, the surface roughness of BC/PTL/Cu significantly increased while the hydrophilicity decreased. Moreover, BC/PTL/Cu displayed slower release rate of Cu compared with BC directly loaded with Cu. BC/PTL/Cu exhibited good antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa. By controlling copper concentration, BC/PTL/Cu were not cytotoxic to mouse fibroblast cell line L929. In vivo, BC/PTL/Cu accelerated wound healing and promoted re-epithelialization, collagen deposition, and angiogenesis while inhibiting inflammation of the infected full-thickness skin wounds of rats. Collectively, these results demonstrate that BC/PTL/Cu composites are promising dressings for healing infected wounds.
在受伤的伤口中，细菌过度生长会导致伤口感染和过度炎症，从而导致伤口愈合延迟。成功治疗延迟感染的伤口愈合需要敷料，这些敷料可以抑制细菌生长和炎症，同时诱导血管生成、胶原蛋白沉积和伤口再上皮化。在这项研究中，制备了细菌纤维素（BC）负载载铜相转变溶菌酶（PTL）纳米膜（BC/PTL/Cu）用于治疗感染性伤口。结果证实，PTL 成功地自组装在 BC 基质上，Cu 通过静电配位加载到 PTL 中。修饰后，膜的拉伸强度和断裂伸长率没有明显变化。与 BC 相比，BC/PTL/Cu 的表面粗糙度显著增加，而亲水性降低。此外，与直接负载 Cu 的 BC 相比，BC/PTL/Cu 显示出较慢的 Cu 释放速率。BC/PTL/Cu 对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和铜绿假单胞菌表现出良好的抗菌活性。通过控制铜浓度，BC/PTL/Cu 对小鼠成纤维细胞系 L929 没有细胞毒性。在体内，BC/PTL/Cu 加速伤口愈合，促进再上皮化、胶原蛋白沉积和血管生成，同时抑制大鼠全层皮肤感染伤口的炎症。总之，这些结果表明，BC/PTL/Cu 复合材料是治疗感染性伤口的有前途的敷料。
