Lee Jong-Hun, Jung Bo Hyun, Yoo Ki-Yeon
Department of Anatomy, College of Dentistry, Gangneung-Wonju National University, 7, Jukheon-gil, Gangneung, 25427, Korea.
Lab Anim Res. 2022 May 8;38(1):12. doi: 10.1186/s42826-022-00121-8.
Transcranial direct current stimulation (tDCS) has been studied as a tool to stimulate the functional recovery of neurons after stroke. Although this device has recently begun to be utilized for providing neuroprotection in stroke, research on its application conditions is lacking. This study aimed to examine the effects of various tDCS application conditions on cerebral ischemia. Ischemia was induced for 5 min in a gerbil model. The application of tDCS comprised a 20 min stimulation-20 min rest-20 min stimulation protocol, which was implemented simultaneously with the induction of cerebral ischemia. Application time of the tDCS effect on ischemia was confirmed by sampling brain tissues after stimulation using 0.2 mA tDCS at 0, 5, 10 and 60 min after ischemia.
Persistence of the tDCS effect on ischemia was confirmed by sampling brain tissues 5, 7, and 10 days post stimulation, with 0.2 mA tDCS after ischemia. Furthermore, the tissues were stained with cresyl violet and Fluoro-Jade C so as to determine the reduction in neuronal death under all application conditions.
The application of tDCS can be used as a useful intervention for acute phase stroke due to its sustained neuroprotective effect.
经颅直流电刺激（tDCS）已被作为一种促进中风后神经元功能恢复的工具进行研究。尽管该设备最近已开始用于中风的神经保护，但对其应用条件的研究仍很缺乏。本研究旨在探讨不同tDCS应用条件对脑缺血的影响。在沙鼠模型中诱导缺血5分钟。tDCS的应用包括一个20分钟刺激-20分钟休息-20分钟刺激的方案，该方案在诱导脑缺血的同时实施。通过在缺血后0、5、10和60分钟使用0.2 mA的tDCS刺激后对脑组织取样，确认tDCS对缺血影响的应用时间。
通过在缺血后使用0.2 mA的tDCS刺激后5、7和10天对脑组织取样，确认了tDCS对缺血影响的持续性。此外，用甲酚紫和氟玉髓C对组织进行染色，以确定在所有应用条件下神经元死亡的减少情况。
由于tDCS具有持续的神经保护作用，其应用可作为急性期中风的一种有效干预措施。
