Faculty of Veterinary Medicine, Razi Universtiy, Kermanshah, Iran.
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Animal Model Exp Med. 2022 Sep;5(3):281-287. doi: 10.1002/ame2.12222. Epub 2022 May 8.
Diabetes-induced oxidative stress can have adverse effects on sperm and its DNA integrity. The Ashrasi date palm (ADP) has potent antioxidant properties. The aim of this study was to evaluate the antioxidant effect of ADP hydroalcoholic extract on sperm parameters and sperm DNA fragmentation in diabetic rats.
Forty male rats were randomly divided into five groups (n = 7): 1, control; 2, diabetic; 3-5, diabetic + ADP (30, 90 and 270 mg/kg for groups 3, 4 and 5, respectively). After preparation of ADP extract and its phytochemical screening, it was administered orally to rats, once a day for 5 weeks. At the end of the study, sperm parameters and sperm DNA fragmentation in all groups were investigated.
At doses of 90 and 270 mg/kg, ADP extract significantly increased the sperm viability compared to diabetic group 2 (p = 0.04 and p = 0.03, respectively) and resulted in a significant decrease in immotile sperm (p = 0.002 and p = 0.006, respectively). At a dose of 270 mg/kg, a considerable enhancement of forward sperm motility was observed (p = 0.04) and there was a significant decrease in sperm DNA fragmentation (p = 0.04).
The findings of the present study show for the first time that the hydroalcoholic extract of ADP has protective and antioxidant effects against diabetes-induced oxidative stress and can improve sperm parameters and protect sperm DNA integrity.
糖尿病引起的氧化应激会对精子及其 DNA 完整性产生不良影响。海椰枣（ADP）具有很强的抗氧化特性。本研究旨在评估 ADP 水醇提取物对糖尿病大鼠精子参数和精子 DNA 碎片化的抗氧化作用。
40 只雄性大鼠随机分为五组（n=7）：1. 对照组；2. 糖尿病组；3-5. 糖尿病+ADP（分别为 30、90 和 270mg/kg 的 3、4 和 5 组）。ADP 提取物制备完成并进行植物化学成分筛选后，大鼠每天口服一次，连续 5 周。研究结束时，检测所有组的精子参数和精子 DNA 碎片化。
在 90 和 270mg/kg 剂量下，ADP 提取物与糖尿病组 2 相比，显著增加了精子活力（p=0.04 和 p=0.03），显著降低了不动精子（p=0.002 和 p=0.006）。在 270mg/kg 剂量下，观察到前向精子活力显著增强（p=0.04），精子 DNA 碎片化显著降低（p=0.04）。
本研究首次表明，ADP 水醇提取物具有抗氧化应激作用，可改善精子参数，保护精子 DNA 完整性。
