Onwuemene Ngozi Joy, Imafidon Christian Eseigbe, Ayoka Abiodun Oladele
Department of Physiological Sciences, Faculty of Basic Medical Sciences Obafemi Awolowo University Ile-Ife Osun State Nigeria.
Department of Physiology, Faculty of Basic Medical and Health Sciences Bowen University Iwo Osun State Nigeria.
Animal Model Exp Med. 2019 Sep 2;2(3):191-200. doi: 10.1002/ame2.12081. eCollection 2019 Sep.
The increasing incidence of chemically induced infertility is both a social threat and a threat to the continuation of life itself. Treatment or management therapy is often expensive. This study investigated the effects of acetone extract of a local plant () in a Wistar rat model of cimetidine-induced pituitary-testicular dysfunction.
Thirty-five male Wistar rats were divided into 7 groups of 5 rats. After a phytochemical screening of an acetone extract of , cimetidine and the extract at three doses, 200, 400 and 600 mg/kg, were orally co-administered to the rats for 28 consecutive days. Comparisons were made (at < 0.05) against a control (2 mL/kg distilled water), a standard treatment group (cimetidine + 50 mg/kg vitamin C), a toxic group (60 mg/kg cimetidine) and a group receiving extract alone.
Cimetidine administration was associated with deleterious alterations to sperm motility, sperm count and sperm viability, as well as derangements in the plasma levels of FSH, LH and testosterone ( < 0.05). Both brain and testicular GSH and TBARS levels were significantly altered following cimetidine administration, and distortions were seen in the pituitary and testicular histoarchitecture. These changes were significantly normalized by co-administration of graded doses of the extract, with an associated improvement of both pituitary and testicular histology.
Acetone extract of normalized cimetidine-induced pituitary-testicular dysfunction in Wistar rats. This presents the extract as a potential nutraceutical choice against chemically induced reproductive toxicity.
化学诱导不孕症发病率的上升对社会构成威胁，也威胁到生命的延续。治疗或管理疗法通常费用高昂。本研究在西咪替丁诱导的垂体 - 睾丸功能障碍的Wistar大鼠模型中，研究了一种当地植物的丙酮提取物的作用。
将35只雄性Wistar大鼠分为7组，每组5只。在对该植物的丙酮提取物进行植物化学筛选后，将西咪替丁和三种剂量（200、400和600mg/kg）的提取物连续28天口服共同给予大鼠。与对照组（2mL/kg蒸馏水）、标准治疗组（西咪替丁+50mg/kg维生素C）、毒性组（60mg/kg西咪替丁）和仅接受提取物的组进行比较（P<0.05）。
给予西咪替丁与精子活力、精子数量和精子存活率的有害改变相关，以及促卵泡激素、促黄体生成素和睾酮血浆水平的紊乱（P<0.05）。给予西咪替丁后，脑和睾丸的谷胱甘肽（GSH）和丙二醛（TBARS）水平均发生显著改变，垂体和睾丸组织结构出现扭曲。通过共同给予分级剂量的提取物，这些变化得到显著正常化，垂体和睾丸组织学也随之改善。
该植物的丙酮提取物使西咪替丁诱导的Wistar大鼠垂体 - 睾丸功能障碍正常化。这表明该提取物是对抗化学诱导生殖毒性的潜在营养保健品选择。
