大肠杆菌染色体polA-metB区域的缺失图谱分析
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
作者信息
Pahel G, Bloom F R, Tyler B
出版信息
J Bacteriol. 1979 May;138(2):653-6. doi: 10.1128/jb.138.2.653-656.1979.
Abstract
A lambdacI857 prophage inserted into one of the genes of the rha locus was used to select deletions unambiguously ordering the markers polA-glnA-rha-pfkA-tpi-metBJF. Transduction with phage P1 indicates at least 70% linkage between glnA and polA. The order of the pfk and tpi markers is reversed from that previously published. Despite the relatively large distance separating the glnA and rha loci, deletions removing this entire region have no obvious phenotype. The isolation of Tn10 transposons integrated at different sites between rha and glnA greatly facilitated this work.
摘要
一个插入到rha位点其中一个基因中的λcI857原噬菌体被用于明确选择缺失突变，从而对标记polA-glnA-rha-pfkA-tpi-metBJF进行排序。用噬菌体P1进行转导表明glnA和polA之间至少有70%的连锁。pfk和tpi标记的顺序与之前发表的相反。尽管glnA和rha位点之间的距离相对较大，但去除整个该区域的缺失突变没有明显的表型。在rha和glnA之间不同位点整合的Tn10转座子的分离极大地促进了这项工作。
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