使用Mu-Mud1双溶原菌确定大肠杆菌谷氨酰胺合成酶基因（glnA）区域转录方向并产生缺失的通用方法。
General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.
作者信息
MacNeil D
出版信息
J Bacteriol. 1981 Apr;146(1):260-8. doi: 10.1128/jb.146.1.260-268.1981.
Abstract
A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method can generate a series of strains containing deletions with predetermined endpoints, and strains with duplications of the region containing the Mud1 insertion. In Escherichia coli, the glnA and glnG genes are transcribed in the same direction.
摘要
本文介绍了一种用于确定细菌基因转录方向的通用遗传学技术。通过比较Mu-Mud1双溶原菌的表型与含缺失衍生物的表型，可以明确确定含有Mud1的基因的转录方向。该方法可以产生一系列含有具有预定端点的缺失的菌株，以及含有Mud1插入区域重复的菌株。在大肠杆菌中，glnA和glnG基因以相同方向转录。
相似文献
1
General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.
J Bacteriol. 1981 Apr;146(1):260-8. doi: 10.1128/jb.146.1.260-268.1981.
2
Fine-structure deletion map and complementation analysis of the glnA-glnL-glnG region in Escherichia coli.
J Bacteriol. 1982 Jun;150(3):1302-13. doi: 10.1128/jb.150.3.1302-1313.1982.
3
Complex glnA-glnL-glnG operon of Escherichia coli.
J Bacteriol. 1982 Apr;150(1):202-13. doi: 10.1128/jb.150.1.202-213.1982.
4
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3743-7. doi: 10.1073/pnas.78.6.3743.
5
Polarity in the glnA operon: suppression of the reg- phenotype by rho mutations.
J Bacteriol. 1982 Jun;150(3):1314-21. doi: 10.1128/jb.150.3.1314-1321.1982.
6
Regulation of expression from the glnA promoter of Escherichia coli in the absence of glutamine synthetase.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7372-6. doi: 10.1073/pnas.77.12.7372.
7
A new glnA-linked regulatory gene for glutamine synthetase in Escherichia coli.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4544-8. doi: 10.1073/pnas.76.9.4544.
8
glnF-lacZ fusions in Escherichia coli: studies on glnF expression and its chromosomal orientation.
Mol Gen Genet. 1984;195(1-2):228-33. doi: 10.1007/BF00332751.
9
Deletion mapping of the polA-metB region of the Escherichia coli chromosome.
J Bacteriol. 1979 May;138(2):653-6. doi: 10.1128/jb.138.2.653-656.1979.
10
Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.
J Bacteriol. 1985 Dec;164(3):1386-9. doi: 10.1128/jb.164.3.1386-1389.1985.
引用本文的文献
1
Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds.
BMC Plant Biol. 2010 Aug 19;10:183. doi: 10.1186/1471-2229-10-183.
2
Control of AmtB-GlnK complex formation by intracellular levels of ATP, ADP, and 2-oxoglutarate.
J Biol Chem. 2010 Oct 1;285(40):31037-45. doi: 10.1074/jbc.M110.153908. Epub 2010 Jul 18.
3
Mapping and expression of a regulatory nitrogen fixation gene (fixD) of Rhizobium meliloti.
EMBO J. 1985 Nov;4(11):2751-6. doi: 10.1002/j.1460-2075.1985.tb03999.x.
4
In vivo functional characterization of the Escherichia coli ammonium channel AmtB: evidence for metabolic coupling of AmtB to glutamine synthetase.
Biochem J. 2005 Aug 15;390(Pt 1):215-22. doi: 10.1042/BJ20042094.
5
Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids.
Plant Physiol. 2003 May;132(1):390-9. doi: 10.1104/pp.102.016675.
6
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
7
The FixK2 protein is involved in regulation of symbiotic hydrogenase expression in Bradyrhizobium japonicum.
J Bacteriol. 1998 Jun;180(12):3253-6. doi: 10.1128/JB.180.12.3253-3256.1998.
8
Modulation of the function of the signal receptor domain of XylR, a member of a family of prokaryotic enhancer-like positive regulators.
J Bacteriol. 1998 Feb;180(3):600-4. doi: 10.1128/JB.180.3.600-604.1998.
9
Heteromeric assembly of the cytosolic glutamine synthetase polypeptides of Medicago truncatula: complementation of a glnA Escherichia coli mutant with a plant domain-swapped enzyme.
Plant Mol Biol. 1997 Nov;35(5):623-32. doi: 10.1023/a:1005884304303.
10
Evidence for two possible glnB-type genes in Herbaspirillum seropedicae.
J Bacteriol. 1997 Jul;179(14):4623-6. doi: 10.1128/jb.179.14.4623-4626.1997.
本文引用的文献
1
Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.
J Bacteriol. 1981 Jan;145(1):348-57. doi: 10.1128/jb.145.1.348-357.1981.
2
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
3
Positive selection for loss of tetracycline resistance.
J Bacteriol. 1980 Aug;143(2):926-33. doi: 10.1128/jb.143.2.926-933.1980.
4
5
Direct selection for P1-sensitive mutants of enteric bacteria.
J Bacteriol. 1974 Jun;118(3):810-4. doi: 10.1128/jb.118.3.810-814.1974.
6
Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes.
J Biol Chem. 1971 Oct 25;246(20):6288-96.
7
Molecular biology of bacteriophage mu.
Science. 1975 Nov 14;190(4215):624-32. doi: 10.1126/science.1103291.
8
Correction and refinement of the genetic map of bacteriophage Mu.
Virology. 1979 Mar;93(2):320-8. doi: 10.1016/0042-6822(79)90236-8.
9
Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.
J Bacteriol. 1978 Nov;136(2):477-83. doi: 10.1128/jb.136.2.477-483.1978.
10
Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.
J Bacteriol. 1978 Oct;136(1):253-66. doi: 10.1128/jb.136.1.253-266.1978.
Zotero 插件