Sugimoto H, Franks D J, Lecavalier L, Chiasson J L, Hamet P
Diabetes. 1987 May;36(5):667-72. doi: 10.2337/diab.36.5.667.
Proliferation of vascular smooth muscle is thought to be involved in the major diabetic complication atherosclerosis. We have previously reported an increase of growth-promoting activity (GA) in platelets from insulin-dependent diabetics. In this study, GA was measured in the platelet extract (PE) from eight diabetic patients who had been treated by conventional insulin therapy. Vascular smooth muscle cells from rat aorta were cultured and used as an assay system for GA. Incorporation of [3H]thymidine into DNA of cultured cells was stimulated by diabetic PE significantly more (P less than .05) than by normal PE. Diabetic PE incubated with cells for 4 days increased cell numbers significantly more (P less than .05) than normal PE. These abnormalities were corrected by long-term intensive insulin treatments (continuous subcutaneous insulin infusion and Pen infuser). The decrease of platelet extract GA appeared to correlate with the amount of insulin administered before meals as short-acting boluses, whereas the level of basal or long-acting insulin appeared to correlate with an increase of PE GA. Thus, the growth-promoting potential of platelets can be normalized by intensive insulin therapy. The relationship of insulin levels to this activity needs further evaluation.
血管平滑肌的增殖被认为与糖尿病主要并发症动脉粥样硬化有关。我们之前报道过胰岛素依赖型糖尿病患者血小板中促生长活性（GA）增加。在本研究中，对8名接受常规胰岛素治疗的糖尿病患者的血小板提取物（PE）中的GA进行了测量。培养大鼠主动脉的血管平滑肌细胞，并将其用作GA的检测系统。糖尿病患者的PE刺激培养细胞DNA中[3H]胸腺嘧啶核苷的掺入，其刺激程度明显高于正常PE（P<0.05）。与细胞一起孵育4天的糖尿病患者PE使细胞数量增加的幅度明显大于正常PE（P<0.05）。这些异常通过长期强化胰岛素治疗（持续皮下胰岛素输注和胰岛素泵）得到纠正。血小板提取物GA的降低似乎与餐前作为短效推注给药的胰岛素量相关，而基础胰岛素或长效胰岛素水平似乎与PE GA的增加相关。因此，强化胰岛素治疗可使血小板的促生长潜能恢复正常。胰岛素水平与这种活性的关系需要进一步评估。
