Barbe P, Salles J P, Barthe P, Louvet J P, Chap H
Department of Endocrinology, CHU Purpan, Toulouse, France.
Mol Cell Biochem. 1992 Feb 12;109(2):167-72. doi: 10.1007/BF00229772.
In order to assess the potential role of the plasma membrane sodium-proton (Na+/H+) exchanger in the pathogenesis of diabetic nephropathy, we investigated 32 insulin dependent (type 1) diabetic patients and 21 control subjects. We tested the Na+/H+ exchange as the rate of amiloride sensitive and sodium dependent volume gain of platelets suspended in sodium propionate. Patients with diabetic nephropathy had significantly increased rates of Na+/H+ exchange (0.31 +/- 0.06 s-1 x 10(-2)) when compared to those without nephropathy (0.24 +/- 0.07, p less than 0.05) or to a control group (0.23 +/- 05, p less than 0.05). Nine patients who were classified as hypertensive had a highly significant increase in the Na+/H+ exchange rates when compared to 23 non-hypertensive diabetic patients: 0.33 +/- 0.04 versus 0.24 +/- 0.06 (p less than 0.001). There was no significant correlation between the Na+/H+ exchange rates and age, diabetes duration, glycated hemoglobin or fructosamine levels on the day of the test. In summary, the data presented here demonstrate an increase in the Na+/H+ exchange rate in insulin-dependent diabetic patients with nephropathy and hypertension.
为了评估质膜钠-质子（Na+/H+）交换体在糖尿病肾病发病机制中的潜在作用，我们研究了32例胰岛素依赖型（1型）糖尿病患者和21名对照受试者。我们以悬浮于丙酸钠中的血小板对amiloride敏感且依赖钠的体积增加率来检测Na+/H+交换。与无肾病的患者（0.24±0.07）或对照组（0.23±0.05）相比，糖尿病肾病患者的Na+/H+交换率显著升高（0.31±0.06 s-1×10(-2)）（p<0.05）。与23例非高血压糖尿病患者相比，9例被归类为高血压的患者的Na+/H+交换率显著升高：0.33±0.04对0.24±0.06（p<0.001）。在检测当天，Na+/H+交换率与年龄、糖尿病病程、糖化血红蛋白或果糖胺水平之间无显著相关性。总之，此处呈现的数据表明，患有肾病和高血压的胰岛素依赖型糖尿病患者的Na+/H+交换率升高。
