用于检测和细胞内成像过氧亚硝酸盐的长波长荧光硼酸酯探针。
Long-wavelength fluorescent boronate probes for the detection and intracellular imaging of peroxynitrite.
作者信息
Sedgwick Adam C, Han Hai-Hao, Gardiner Jordan E, Bull Steven D, He Xiao-Peng, James Tony D
机构信息
Department of Chemistry, University of Bath, Bath, BA2 7AY, UK.
出版信息
Chem Commun (Camb). 2017 Nov 28;53(95):12822-12825. doi: 10.1039/c7cc07845e.
Two boronate fluorescent probes have been developed for the detection of peroxynitrite (TCFB1 and TCFB2). TCFB1 was shown to have a low sensitvity towards peroxynitrite and have a poor solubility in aqueous solution whereas TCFB2 demonstrated high sensitivity towards peroxynitrite and mitochondria localisation with the ability to detect exogenous and endogenous peroxynitrite in live cells (Hep-G2, RAW 264.7, HeLa and A459).
已经开发出两种用于检测过氧亚硝酸盐的硼酸酯荧光探针（TCFB1和TCFB2）。结果表明，TCFB1对过氧亚硝酸盐的敏感性较低，在水溶液中的溶解度较差，而TCFB2对过氧亚硝酸盐表现出高敏感性和线粒体定位能力，能够检测活细胞（Hep-G2、RAW 264.7、HeLa和A459）中的外源性和内源性过氧亚硝酸盐。
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