基于一维排列磁等离子体纳米颗粒上石墨烯量子点标记的双模式表面增强拉曼散射-荧光免疫分析
Dual-Mode SERS-Fluorescence Immunoassay Using Graphene Quantum Dot Labeling on One-Dimensional Aligned Magnetoplasmonic Nanoparticles.
作者信息
Zou Fengming, Zhou Hongjian, Tan Tran Van, Kim Jeonghyo, Koh Kwangnak, Lee Jaebeom
机构信息
†High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei, Anhui 230031, P. R. China.
‡Department of Nano Fusion Technology and BK21 Plus Nano Convergence Technology Division, Pusan National University, Busan 609-735, Republic of Korea.
出版信息
ACS Appl Mater Interfaces. 2015 Jun 10;7(22):12168-75. doi: 10.1021/acsami.5b02523. Epub 2015 Jun 1.
A novel dual-mode immunoassay based on surface-enhanced Raman scattering (SERS) and fluorescence was designed using graphene quantum dot (GQD) labels to detect a tuberculosis (TB) antigen, CFP-10, via a newly developed sensing platform of linearly aligned magnetoplasmonic (MagPlas) nanoparticles (NPs). The GQDs were excellent bilabeling materials for simultaneous Raman scattering and photoluminescence (PL). The one-dimensional (1D) alignment of MagPlas NPs simplified the immunoassay process and enabled fast, enhanced signal transduction. With a sandwich-type immunoassay using dual-mode nanoprobes, both SERS signals and fluorescence images were recognized in a highly sensitive and selective manner with a detection limit of 0.0511 pg mL(-1).
基于表面增强拉曼散射（SERS）和荧光的新型双模式免疫分析方法被设计出来，该方法使用石墨烯量子点（GQD）标记，通过新开发的线性排列磁等离子体（MagPlas）纳米颗粒（NP）传感平台来检测结核（TB）抗原CFP-10。GQD是用于同时进行拉曼散射和光致发光（PL）的优异双标记材料。MagPlas NP的一维（1D）排列简化了免疫分析过程，并实现了快速、增强的信号转导。使用双模式纳米探针进行夹心型免疫分析时，SERS信号和荧光图像均以高灵敏度和高选择性被识别，检测限为0.0511 pg mL(-1)。
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