Pan Rui, Buitrago Sebastian, Feng Zhenbao, Abou-Elwafa Salah Fatouh, Xu Le, Li Chengdao, Zhang Wenying
Research Center of Crop Stresses Resistance Technologies/Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, China.
Department of Agronomy, Faculty of Agriculture, Assiut University, Assiut, Egypt.
Front Plant Sci. 2022 Apr 22;13:878459. doi: 10.3389/fpls.2022.878459. eCollection 2022.
Drought stress is a common environmental stress, which adversely affects the yield and quality of crops. Due to its excellent drought tolerance, wild barley from the Middle East region is considered a valuable source for barley improvement. Here, we compared the growth rate, stomatal regulation and capacity to metabolize reactive oxygen species (ROS) of two barley cultivars and one wild barley accession. The results indicated the wild barley EC_S1 showed a more significant decline in stomatal aperture and less ROS production. Transcriptomic analysis revealed that EC_S1 has slower transcriptional regulation (5,050 DEGs) in the early stage of drought stress (14 days) than Baudin (7,022 DEGs) and Tadmor (6,090 DEGs). In addition, 30 hub genes, including nine known drought-related genes were identified by WGCNA analysis. Then, we cloned a novel transcription factor, , from EC_S1. was subcellularly targeted to the nucleus. Overexpression of in enhanced drought tolerance due to increasing activities of superoxide dismutase, peroxidase, and catalase activities as well as glutathione content. Silencing of in EC_S1 suppressed drought tolerance in BSMV:HvbZIP21-inoculated plants. Taken together, our findings suggest that play a critical role in drought tolerance by manipulating ROS scavenging.
干旱胁迫是一种常见的环境胁迫，会对作物的产量和品质产生不利影响。由于其出色的耐旱性，来自中东地区的野生大麦被认为是大麦改良的宝贵资源。在此，我们比较了两个大麦品种和一个野生大麦种质的生长速率、气孔调节以及代谢活性氧（ROS）的能力。结果表明，野生大麦EC_S1的气孔孔径下降更为显著，ROS产生较少。转录组分析显示，在干旱胁迫早期（14天），EC_S1的转录调控（5050个差异表达基因）比Baudin（7022个差异表达基因）和Tadmor（6090个差异表达基因）更慢。此外，通过加权基因共表达网络分析（WGCNA）鉴定出30个枢纽基因，其中包括9个已知的与干旱相关的基因。然后，我们从EC_S1中克隆了一个新的转录因子 。 在亚细胞水平上定位于细胞核。在 中过表达 可增强耐旱性，这是由于超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性以及谷胱甘肽含量增加。在EC_S1中沉默 会抑制接种BSMV:HvbZIP21的植物的耐旱性。综上所述，我们的研究结果表明， 通过调控ROS清除在耐旱性中起关键作用。
