Yuan Junhua, Zhang Di, Wang Yunyang, Zhu Zhen, Lin Qian, Li Manwen, Zhong Weizhen, Han Jing, Xu Fengsen, Dong Jing
Special Medicine Department, School of Basic Medicine, Qingdao University, Qingado, China.
Shandong Provincial Engineering Laboratory of Novel Pharmaceutical Excipients, Sustained and Controlled Release Preparations, College of Medicine and Nursing, Dezhou University, Shandong, China.
Int J Endocrinol. 2022 Apr 26;2022:1113811. doi: 10.1155/2022/1113811. eCollection 2022.
Gestational diabetes mellitus (GDM) poses a significant health risk to pregnant women, and thus exploring the potential underlying mechanism is highly desirable. The aim of the study was to compare maternal serum, cord blood serum, and placental angiopoietin-like 8 (ANGPTL8) levels in the third trimester of pregnancy in women with and without gestational diabetes and explore the potential underlying mechanism. A total of 42 pregnant women (23 with GDM and 19 with normal glucose tolerance (NGT)) along with 29 age-matched non-pregnant healthy females were enrolled. All pregnant subjects were in the late third trimester. Maternal serum and cord blood serum ANGPTL8 levels were measured with an enzyme-linked immunosorbent assay and the protein levels of ANGPTL8 in placentas were assessed with western blotting. The associations between maternal serum and cord blood serum ANGPTL8 levels and metabolic parameters were investigated with the Spearman correlation analysis. Significantly lower levels of maternal serum and placental ANGPTL8 levels were observed in GDM patients compared to NGT pregnant women, while remarkably higher ANGPTL8 levels were present in the cord blood serum samples. The maternal serum ANGPTL8 level was positively correlated with BMI, total cholesterol, triglycerides, and AUC for OGTT and birthweight. Additionally, the cord blood serum ANGPTL8 level was positively correlated with insulin and the homeostatic model assessment for insulin resistance. Both maternal serum and cord blood serum ANGPTL8 levels seemed to correlate with GDM and has the potential to be used as a biomarker for GDM and birthweight prediction.
妊娠期糖尿病（GDM）对孕妇构成重大健康风险，因此探索其潜在的发病机制非常必要。本研究旨在比较患有和未患有妊娠期糖尿病的孕妇在妊娠晚期的母血清、脐血血清和胎盘血管生成素样8（ANGPTL8）水平，并探讨其潜在的发病机制。共纳入42名孕妇（23名患有GDM，19名糖耐量正常（NGT））以及29名年龄匹配的非妊娠健康女性。所有孕妇均处于妊娠晚期。采用酶联免疫吸附测定法检测母血清和脐血血清ANGPTL8水平，并用蛋白质印迹法评估胎盘中ANGPTL8的蛋白水平。采用Spearman相关分析研究母血清和脐血血清ANGPTL8水平与代谢参数之间的关联。与NGT孕妇相比，GDM患者的母血清和胎盘ANGPTL8水平显著降低，而脐血血清样本中的ANGPTL8水平显著升高。母血清ANGPTL8水平与BMI、总胆固醇、甘油三酯、OGTT曲线下面积（AUC）和出生体重呈正相关。此外，脐血血清ANGPTL8水平与胰岛素及胰岛素抵抗稳态模型评估呈正相关。母血清和脐血血清ANGPTL8水平似乎均与GDM相关，并有潜力用作GDM和出生体重预测的生物标志物。
